FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_37.3410000000512a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_37.3410000000512a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1182270
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC40670.34399925566917877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4850.036.64841545
TATGCCG5850.030.76833543
ATGCCGT6000.029.99912844
CCCACGA6500.028.3837919
GCCCACG6550.027.82361818
CTCGTAT6500.027.69150239
TCGTATG6800.027.46243540
CGAGCCC6700.027.200715
TCTCGTA6650.027.0668838
ACGAGAC6900.027.06442822
CCACGAG6850.026.60506620
TATCTCG6150.026.34069636
CACGAGA7250.025.44753521
ATAGCGC450.001226784124.9992718
CGTATGC7300.024.65681641
CCGAGCC7550.024.43637514
CGAGACC6650.024.36019123
TAGCGCA651.5241425E-524.2300629
ACTTATC6350.023.73946633
TACTTAT6450.023.7202432
Produced by FastQC (version 0.11.2)