FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_36.3410000000511d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_36.3410000000511d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550610
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC7967614.470496358584114No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGT101921.8510379397395618No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCG53820.977461361035942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC83400.044.46734245
ATGCCGT93650.039.64124744
TATGCCG99550.037.3144443
GTATGCC99700.037.30343642
CGTATGC99900.037.2963241
TATCTCG99200.037.2646536
CTCGTAT99750.037.23962439
TCGTATG100100.037.221840
TCTCGTA99800.037.22096638
ATCTCGT99650.037.20925537
CTACTTA99600.037.2053531
TACTTAT99750.037.1719632
ACTTATC99650.037.0286333
CACGAGA101400.036.92210821
ACGAGAC100900.036.88208822
CCTCTAC100100.036.83969528
CCCACGA101700.036.83531619
CCACGAG101700.036.83531620
CTTATCT100150.036.821334
ACCTCTA100100.036.81721527