FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_35.34100000005100.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_35.34100000005100.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026835
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCG250.002107026535.99992417
CACGCGG401.5604292E-533.74992817
TATGCCG1450.031.03441643
CGCGCAA300.0051470629.99993520
GACGCGC300.0051470629.99993518
CCCGCAC300.0051470629.99993525
GTTTCGG803.3942342E-928.12493921
ACGCGGG507.1577735E-526.99994318
CCCACGA1800.024.99994719
TCCGTTC1001.3442332E-924.74994716
TGCCGTC1850.024.32427245
TCTCGTA1900.023.6841638
CGCCTAA1003.6434358E-822.49995222
TTCGGGG1003.6434358E-822.49995223
ACACGCG602.467296E-422.4999516
ACGCCTA1104.0508894E-922.4999521
CGTTCCA1104.0508894E-922.4999518
CGCAATC602.467296E-422.4999522
GCCCACG2100.021.42852418
TCGGGGT955.5306555E-721.31574424