FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_34.341000000050f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_34.341000000050f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890691
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCC250.002106738336.0004049
TATGCCG751.7025741E-929.99865343
TGCCGTC901.1908924E-824.99887745
CCGAACG551.3677609E-424.545739
AACCGTC952.1151209E-823.68314734
GTATAAT1252.910383E-1123.4081481
GGTATCA4150.023.3213711
CGCCTAA1003.6416168E-822.50025422
ATCTCCG7900.022.49899145
CCGTCTC1204.5110937E-1022.49899136
GTATCAA7250.022.0421561
ACGCCTA1056.103255E-821.42881221
CATCTCC23250.021.38613745
CGAGCCC955.5312375E-721.31483315
GACCGAA654.236878E-420.7694637
CTACGCT550.00393687720.4536274
GTCTTAT1503.4197E-1019.506791
TACTATA951.3151606E-518.9475822
GCGTAAG600.006496301418.7565291
GCCCACG1202.496272E-718.74915918