FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_32.341000000050d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_32.341000000050d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTC712.8174603174603177No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC471.8650793650793651No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC411.6269841269841272No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC180.7142857142857143RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC180.7142857142857143No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC120.4761904761904762No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAGGCGAATCTCGTATGCCGTC100.3968253968253968No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC90.35714285714285715RNA PCR Primer, Index 13 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC60.2380952380952381No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC40.15873015873015872No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCGCTGATCTCGTATGCCGTC40.15873015873015872No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC30.11904761904761905No Hit
CTTCTGGAGTGTCTGAAGACAGCTACAGTGTACTTACACTGTCTCTTATAC30.11904761904761905No Hit
ACATAGGCTTCATTAAGTTGGACTAAGCGACCTTGAATGGATTCGACTGAC30.11904761904761905No Hit
CTTATACACATCTCCGAGCCCACGAGAACTCTACTTATCTCGTATGCCGTC30.11904761904761905No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTACTTATCTCGTATGCCGTCT30.11904761904761905No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT30.11904761904761905RNA PCR Primer, Index 44 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCC354.106454E-638.5714315
CCGAGCC354.106454E-638.5714314
AGCCCAC308.466285E-537.50000417
GCCCACG308.466285E-537.50000418
TGCCGTC308.466285E-537.50000445
CCACGAG308.466285E-537.50000420
GAGCCCA308.466285E-537.50000416
CACGAGA308.466285E-537.50000421
CCCACGA308.466285E-537.50000419
ACGAGAC308.466285E-537.50000422
TATCTCG308.466285E-537.50000436
ATCTCCG401.0283513E-533.7510
TCTCCGA401.0283513E-533.7511
TCCGAGC401.0283513E-533.7513
CTCCGAG401.0283513E-533.7512
GTATGCC352.0993862E-432.1428642
ATCTCGT352.0993862E-432.1428637
TATGCCG352.0993862E-432.1428643
TCTCGTA352.0993862E-432.1428638
ATGCCGT352.0993862E-432.1428644