FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_31.341000000050cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_31.341000000050cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6854
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC851.2401517362124308No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC580.8462211847096587No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC440.6419608987452583No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC430.6273708783192297No Hit
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC410.5981908374671724RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC340.4960606944849723No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC330.4814706740589437No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC310.45229063320688645No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC310.45229063320688645RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC180.2626203676685147RNA PCR Primer, Index 13 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC180.2626203676685147No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC170.24803034724248615No Hit
CTTATACACATCTCCGAGCCCACGAGACCATTCCGAATCTCGTATGCCGTC160.23344032681645752RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCAACTGTATCTCGTATGCCGTC150.21885030639042896No Hit
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT140.20426028596440038No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC140.20426028596440038No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC130.18967026553837174No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC130.18967026553837174RNA PCR Primer, Index 23 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC100.14590020426028597No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT100.14590020426028597No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCG90.13131018383425735No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCTATGATCTCGTATGCCGTC80.11672016340822876No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC70.10213014298220019No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC70.10213014298220019No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC650.041.53846445
GTATGCC700.038.57142642
TATGCCG700.038.57142643
ATGCCGT700.038.57142644
CGTATGC700.038.57142641
TCGTATG700.038.57142640
AAATCTC250.001964555636.00000435
CGAGACA250.001964555636.00000423
ATCTCGT750.036.037
TCTCGTA750.036.038
CGAGCCC750.036.015
CCGAGCC750.036.014
TCCGAGC750.036.013
CTCGTAT750.036.039
CTCCGAG750.036.012
AATCTCG457.1456634E-735.00000436
AGCCCAC801.8189894E-1233.7517
GCCCACG801.8189894E-1233.7518
CCACGAG801.8189894E-1233.7520
CCCACGA801.8189894E-1233.7519
Produced by FastQC (version 0.11.2)