FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_30.341000000050be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_30.341000000050be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693823
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC10605215.285166389698816RNA PCR Primer, Index 13 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGT166352.3975855513581994No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCG63190.9107510128663938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC113600.044.4825445
ATGCCGT130600.038.74399644
TATGCCG137700.036.8443443
ATCTCGT137500.036.73430637
CTCGTAT137600.036.72395739
GTATGCC138250.036.71403542
CGTATGC138050.036.70203441
TCGTATG138100.036.67245540
AATCTCG137000.036.6548836
TCTCGTA137800.036.6543338
ACGAGAC139100.036.60291322
CCCACGA139550.036.59773319
GCCCACG139600.036.5737818
CCACGAG139650.036.57152620
CGAGACC139100.036.5705623
CGAGCCC139850.036.5619415
CACGAGA139700.036.5101221
GAGCCCA140000.036.5066916
AGACCTA138800.036.5037225
AGCCCAC139950.036.50102217
Produced by FastQC (version 0.11.2)