FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_3.34100000004ea7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_3.34100000004ea7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284191
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2450.037.659541
ACGGGCC300.00514091429.99988217
CTAGTGT300.00514091429.9998824
TATTCCG300.00514091429.9998825
AGAATAG406.154773E-428.1248915
AGTAGGG507.139961E-526.9998935
GTATCAA5950.026.4751421
TAGAGTA500.002260522.499915
GAGTGCC500.002260522.499917
AACTGAG500.002260522.499917
GAACTGA654.2275334E-420.7691486
CTACTAA550.003925444620.4580671
ATATTCC550.003929449220.4544664
ACTGACC807.5376716E-519.6874248
CTAATAT706.9577113E-419.2890341
TATCAAC8350.018.8622022
TACTTAC1709.094947E-1118.52933931
ACGGGGT750.001107458717.9999316
TAGAAGT901.8595038E-417.4999314
ATCAACG9150.017.2130493
Produced by FastQC (version 0.11.2)