FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_29.341000000050a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_29.341000000050a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1720034
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC82200.47789752993254786RNA PCR Primer, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9150.040.57670645
GTATGCC11300.033.451842
ATGCCGT11300.033.25268644
TATGCCG11600.032.392743
ACGAGAC11950.032.00789322
CTCGTAT12050.031.1821139
ACCTATT10550.031.13697827
GCCCACG12350.030.78901718
CGTATGC12350.030.42553541
CCCACGA12700.030.29482519
ATCTCGT12550.030.11907437
TCGTATG12600.029.99955440
TCTCGTA12800.029.35503238
GACCTAT11300.029.26947626
AGACCTA11700.028.26881225
TATTCAA11700.028.07650830
TGGACCG654.8764014E-727.6918955
CCACGAG14150.027.1904120
CGAGACC12500.027.17959623
AGCCCAC14000.027.16031317
Produced by FastQC (version 0.11.2)