FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_28.34100000005095.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_28.34100000005095.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571774
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTATTCAAATCTCGTATGCCGTC50990.32441050685403877RNA PCR Primer, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7050.041.48971645
TATGCCG8550.034.2097343
GCCCACG9200.032.28288318
CCCACGA9350.031.76396619
CGAGCCC9500.031.7371115
AATCTCG8300.031.17397336
CCACGAG9600.031.17114820
CTCGTAT9500.030.78875739
ATGCCGT9700.030.15393644
CGAGACC9100.029.91688323
CCGAGCC10100.029.85173614
TCTCGTA9900.029.54476538
ACGAGAC10250.029.19443922
CACGAGA10550.028.36426721
TCGTATG11000.027.20390940
ATCTCGT10900.026.83423837
ACCTATT9500.026.76423627
GAGCCCA11500.026.0219616
AGCCCAC11600.025.79763217
GTAGACG801.0545409E-725.31190919
Produced by FastQC (version 0.11.2)