FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_27.34100000005088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_27.34100000005088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149737
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1650.029.999845
GGTATCA5800.026.7752111
TATGCCG2000.024.74983243
ATGCCGT2000.024.74983244
GTATCAA12050.024.4678631
CCGTACG602.4676233E-422.49984733
TCTCCGA10000.020.24986345
CATCTCC39050.020.108745
CTCGCAA908.2517345E-619.99986616
ACCTATT2200.019.43168627
TCTCGTA2550.019.41163438
GCCCACG2450.019.28558318
CGTCGTG706.9837907E-419.28558228
TCGTATG2600.019.03833440
TAGCGCA600.00651075318.7498749
AGCTCGC600.00651075318.74987410
CTCGTAT2700.018.3332139
CCACGAG2850.018.15777220
CCCACGA3000.017.99987819
AGGGCGA750.00111027417.99987810