FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_26.3410000000507b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_26.3410000000507b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152723
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCG300.005147676429.999519
TGCCGTC1350.024.99959245
GGTATCA4450.024.2797971
CATCTCC29250.023.15346545
TATACTA702.7033446E-522.499635
ATCTCCG9650.020.9841145
TATGCCG1653.6379788E-1220.45420843
GCCCACG1703.6379788E-1219.85261518
CGAGCCC1703.6379788E-1219.85261515
CGGGTGG600.006511122518.74969317
CGTCCAG600.006511122518.74969310
GTACTTA5850.018.46203630
CCCACGA1951.8189894E-1218.46123719
ACGTTAT750.001107120218.0075171
GTATCAA9350.017.8149221
CCACGAG2150.017.79040520
TACTTAC5950.017.7728231
AGTGTAC7300.017.56896827
CGTGCAA901.8659167E-417.49971211
ACAGCGC901.8659167E-417.4997128
Produced by FastQC (version 0.11.2)