##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 18h_25.3410000000506e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 64458 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.28837382481616 38.0 38.0 38.0 32.0 38.0 2 36.245260479692206 38.0 38.0 38.0 32.0 38.0 3 36.1928077197555 38.0 38.0 38.0 32.0 38.0 4 36.27484563591796 38.0 38.0 38.0 32.0 38.0 5 36.29237643116448 38.0 38.0 38.0 32.0 38.0 6 38.19448322938968 40.0 38.0 40.0 38.0 40.0 7 38.092059946011354 40.0 38.0 40.0 38.0 40.0 8 38.0105495051041 40.0 38.0 40.0 32.0 40.0 9 38.04589034720283 40.0 38.0 40.0 32.0 40.0 10 37.93848707685625 40.0 38.0 40.0 32.0 40.0 11 37.959198237612085 40.0 38.0 40.0 32.0 40.0 12 37.88254367184834 40.0 38.0 40.0 32.0 40.0 13 37.918241335443234 40.0 38.0 40.0 32.0 40.0 14 37.90570604114307 40.0 38.0 40.0 32.0 40.0 15 37.90767631636104 40.0 38.0 40.0 32.0 40.0 16 37.892007198485835 40.0 38.0 40.0 32.0 40.0 17 37.9010208197586 40.0 38.0 40.0 32.0 40.0 18 37.91647274194049 40.0 38.0 40.0 32.0 40.0 19 37.92004095690217 40.0 38.0 40.0 32.0 40.0 20 37.8766949021068 40.0 38.0 40.0 32.0 40.0 21 37.920614974091656 40.0 38.0 40.0 32.0 40.0 22 37.86313568525241 40.0 38.0 40.0 32.0 40.0 23 37.89039374476403 40.0 38.0 40.0 32.0 40.0 24 37.91034472059325 40.0 38.0 40.0 32.0 40.0 25 37.90579912501163 40.0 38.0 40.0 32.0 40.0 26 37.853377393031124 40.0 38.0 40.0 32.0 40.0 27 37.78894784200565 40.0 38.0 40.0 32.0 40.0 28 37.76628192000993 40.0 38.0 40.0 32.0 40.0 29 37.72527847590679 40.0 38.0 40.0 32.0 40.0 30 37.68242886841044 40.0 38.0 40.0 32.0 40.0 31 37.57884203667504 40.0 38.0 40.0 32.0 40.0 32 37.44387042725496 40.0 38.0 40.0 32.0 40.0 33 37.34040770734432 40.0 38.0 40.0 32.0 40.0 34 37.31761767352385 40.0 38.0 40.0 32.0 40.0 35 37.20616525489466 40.0 38.0 40.0 32.0 40.0 36 37.069021688541376 40.0 38.0 40.0 32.0 40.0 37 36.98802320891123 40.0 38.0 40.0 32.0 40.0 38 36.867324459337866 40.0 38.0 40.0 32.0 40.0 39 36.72600763287722 40.0 38.0 40.0 32.0 40.0 40 36.579059232368365 40.0 38.0 40.0 27.0 40.0 41 36.41110490551987 40.0 38.0 40.0 27.0 40.0 42 36.19932359055509 40.0 38.0 40.0 27.0 40.0 43 36.003661298830245 40.0 38.0 40.0 27.0 40.0 44 35.7963635235347 38.0 38.0 40.0 27.0 40.0 45 35.560830308107604 38.0 38.0 40.0 13.0 40.0 46 35.28370411740978 38.0 38.0 40.0 13.0 40.0 47 35.024884420863195 38.0 38.0 40.0 13.0 40.0 48 34.76241893946446 38.0 38.0 40.0 13.0 40.0 49 34.5919358341866 38.0 32.0 40.0 13.0 40.0 50 34.45178255608303 38.0 32.0 40.0 13.0 40.0 51 33.79470352787862 38.0 32.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.14420230317004012 2201 2 0.4147272709273153 2201 3 0.6238484534925064 2201 4 0.26318910104100013 2201 5 -0.19925024996167906 2201 6 0.9446410932399445 2201 7 -0.22162389500605428 2201 8 -0.18623957946800118 2201 9 0.33987643714326055 2201 10 0.8293019407021447 2201 11 -0.11095037510489902 2201 12 0.31129873909274863 2201 13 -0.22560084101863254 2201 14 -0.4679984278513061 2201 15 -0.05307175089750871 2201 16 -0.45654811517797356 2201 17 -0.669109123644624 2201 18 -0.48237113300111645 2201 19 0.2510392620794306 2201 20 0.46063395022323306 2201 21 0.21723215030306164 2201 22 0.8210624439560377 2201 23 -0.14009796072177494 2201 24 0.47214689316847824 2201 25 0.5744965169489333 2201 26 0.3715175150861185 2201 27 0.46316337964520926 2201 28 -0.3170436664212275 2201 29 0.3036428192309373 2201 30 -0.4849133830739021 2201 31 -0.4403949426438345 2201 32 -0.5019271905434266 2201 33 -0.8924471087814325 2201 34 0.03767126375244345 2201 35 -0.25148095302981943 2201 36 -0.7524072630968917 2201 37 0.09986901308141682 2201 38 0.015283732979135323 2201 39 0.7747308344850197 2201 40 0.6300990745132609 2201 41 1.0492201980346465 2201 42 0.9749209339801581 2201 43 0.9435589495868726 2201 44 1.5305676971152593 2201 45 1.3232322083809436 2201 46 0.7924818902010244 2201 47 1.089693516289259 2201 48 0.5630585926476286 2201 49 0.46889673297739876 2201 50 1.0967503467549022 2201 51 1.121811368669718 1101 1 0.09479331971850513 1101 2 0.008580698823294597 1101 3 -0.16598606187392306 1101 4 -0.25903312118122557 1101 5 -0.29783181024536987 1101 6 0.24936922562765318 1101 7 0.40532645960387725 1101 8 0.5983939902719513 1101 9 0.4084343567650137 1101 10 -0.06455148719383175 1101 11 0.12096451851212464 1101 12 0.434702994411893 1101 13 0.3774724450333764 1101 14 0.0038691844418394794 1101 15 0.5086303767620706 1101 16 0.5056268257203769 1101 17 -0.1683999037864723 1101 18 0.5963522712542044 1101 19 0.4465475363111082 1101 20 -0.3601698322826792 1101 21 0.3526931432108569 1101 22 -0.177755522947038 1101 23 -0.31480245244754457 1101 24 0.052761550378882305 1101 25 0.1822979353886467 1101 26 0.39823146307666235 1101 27 0.6220286278721616 1101 28 0.8888664990634041 1101 29 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1213 32 -0.5789780732017888 1213 33 -2.2679403590410416 1213 34 -2.2337721422704035 1213 35 -1.5995598730713567 1213 36 -1.2860521229099717 1213 37 -0.7028308830764232 1213 38 -1.2532520925660364 1213 39 -1.3769825611536106 1213 40 -0.9588936565355439 1213 41 -1.918381048071275 1213 42 -0.4691081418246199 1213 43 -1.308050603891843 1213 44 -1.3675631440062332 1213 45 -1.0650336275484449 1213 46 -2.1539355552818407 1213 47 -1.0097872729111614 1213 48 -0.8563598912568366 1213 49 -1.5775207125054678 1213 50 -1.4006535992471782 1213 51 -0.6948033769169939 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 3.0 16 4.0 17 3.0 18 5.0 19 7.0 20 13.0 21 24.0 22 31.0 23 93.0 24 163.0 25 261.0 26 405.0 27 642.0 28 774.0 29 1183.0 30 1357.0 31 1643.0 32 2086.0 33 2586.0 34 3142.0 35 3605.0 36 4333.0 37 5880.0 38 9204.0 39 27010.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.168042709044634 12.542678006083555 12.221429014836428 23.067850270035382 2 18.007353739702438 22.911398296538778 38.93138061032937 20.149867353429418 3 21.887120295386143 28.26646808774706 29.934220732880323 19.912190883986472 4 14.806540693164541 15.998014210803934 34.368115672220675 34.82732942381085 5 14.947717893822334 35.15002016817152 29.180241397499145 20.722020540506996 6 30.466350181513548 34.28278879270222 13.926898135219833 21.3239628905644 7 27.33407800428186 35.163982748456355 20.435011945763133 17.066927301498648 8 28.317664215458127 29.319867200347517 17.952775450681063 24.409693133513297 9 33.417108815042354 13.83226286884483 18.674175432064292 34.07645288404853 10 21.54581277731236 25.486363213255142 26.359800179962146 26.60802382947035 11 32.33678788631004 23.619213106615366 22.047598361673078 21.996400645401515 12 28.63570076639052 24.183189053337056 25.32346644326538 21.85764373700704 13 30.5284060938906 23.542461758044 24.52915076483912 21.399981383226287 14 26.5754444754724 22.53715597753576 27.940674547767543 22.9467249992243 15 25.238139563746937 26.84383629650315 28.41229948183313 19.505724657916783 16 19.15415166935584 29.899466302594018 28.02997393570808 22.916408092342063 17 22.422700404921112 23.45284453201359 24.766898862807764 29.357556200257534 18 24.817710757392412 23.627788637562443 27.50007756989047 24.054423035154674 19 30.435322225325017 21.446523317509076 28.080300350615907 20.03785410655 20 26.786434577554374 20.86009494554594 27.957739923671227 24.39573055322846 21 26.775574792888392 22.56663253591486 30.688200068261505 19.969592602935244 22 24.48726302398461 21.328617083992675 29.788389338794254 24.39573055322846 23 24.350740016755097 24.16612367743337 31.04036737100127 20.442768934810264 24 22.642651028576747 24.287132706568617 31.969654658847624 21.100561606007012 25 21.22622482857054 24.000124111824757 32.00999100189271 22.763660057712 26 19.18923950479382 25.72217568028794 33.085109683825124 22.003475131093115 27 19.300940147072513 26.490117595953954 32.46455056005461 21.744391696918925 28 18.467839523410593 28.167178627943777 28.66517732476962 24.69980452387601 29 19.705854975332777 29.94973471097459 29.921809550404916 20.422600763287722 30 22.697654213727194 26.525071366513593 28.472136030780685 22.30513838897853 31 22.188091470414843 26.445127059480594 29.74029600670204 21.626485463402524 32 22.4626888826833 25.36225138850104 29.692202674609824 22.48285705420584 33 19.809798628564337 24.415898724751 30.28483663781067 25.489466008873997 34 19.83306959570573 27.858450463867946 32.34354153091935 19.964938409506967 35 20.998169350584877 29.183344193118 29.009587638462254 20.80889881783487 36 19.964938409506967 28.424710664308545 29.968351484687705 21.64199944149679 37 18.07223308200689 30.62769555369388 31.425114027738992 19.87495733656024 38 19.375407241924975 28.660523131341336 29.544819882714325 22.41924974401936 39 16.626330323621584 30.11728567439263 31.634552732011546 21.621831269974244 40 19.496416271060223 26.49477178938223 31.693505848769743 22.3153060907878 41 18.80294145024667 27.860001861677368 31.778832728288187 21.55822395978777 42 19.41419218716063 27.46284402246424 31.95879487418164 21.16416891619349 43 17.859691582115484 26.673182537466257 30.106425889726644 25.360699990691614 44 15.411585838840796 26.96949951906668 32.95634366564275 24.662570976449782 45 14.552111452418629 27.493871978652766 33.18129634800956 24.772720220919048 46 14.879456390207576 25.27382171336374 32.41180303453412 27.43491886189457 47 13.771758354277203 26.18759502311583 32.95479226783332 27.08585435477365 48 14.82360606906823 27.05172360296627 31.252908870892675 26.871761457072825 49 15.498464116168668 27.03155543144373 31.311861987650875 26.15811846473673 50 15.600856371590805 27.951534332433525 30.356200937044274 26.091408358931396 51 15.486052933693259 26.950882745353567 28.438673244593378 29.1243910763598 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3.0 1 2.0 2 1.0 3 0.5 4 0.0 5 2.5 6 5.0 7 6.5 8 8.0 9 16.0 10 24.0 11 75.5 12 127.0 13 329.0 14 531.0 15 876.0 16 1221.0 17 1466.5 18 1712.0 19 1673.0 20 1634.0 21 1527.5 22 1421.0 23 1488.0 24 1555.0 25 1656.0 26 1371.0 27 985.0 28 796.5 29 608.0 30 572.0 31 536.0 32 991.0 33 1446.0 34 1709.5 35 1973.0 36 1749.0 37 1525.0 38 1350.5 39 1176.0 40 1384.5 41 1593.0 42 2066.5 43 2540.0 44 3015.5 45 3491.0 46 3785.5 47 4080.0 48 4928.5 49 5777.0 50 5579.5 51 5382.0 52 5467.5 53 5553.0 54 5142.5 55 4732.0 56 4027.0 57 3322.0 58 3133.0 59 2944.0 60 2538.5 61 2133.0 62 1876.5 63 1620.0 64 1318.0 65 1016.0 66 819.5 67 623.0 68 526.0 69 429.0 70 379.5 71 330.0 72 297.0 73 264.0 74 214.5 75 130.0 76 95.0 77 74.0 78 53.0 79 42.0 80 31.0 81 25.5 82 20.0 83 16.5 84 13.0 85 6.5 86 0.0 87 1.5 88 3.0 89 1.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03413075180737845 2 0.0015513978094262929 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0031027956188525857 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0031027956188525857 17 0.0015513978094262929 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0031027956188525857 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 64458.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.82260473648046 #Duplication Level Percentage of deduplicated Percentage of total 1 87.33649427553553 57.48715541768463 2 7.15927013727348 9.42483616894881 3 2.1216128722277396 4.189502564774266 4 0.9570549138473285 2.519833892211163 5 0.4865542289613283 1.6013133347892263 6 0.398782011111178 1.5749322416033897 7 0.25449364627966936 1.1726004280908644 8 0.1746874636628693 0.9198707098479472 9 0.15670026971493908 0.9282977923991684 >10 0.8646291935227025 11.205219673816739 >50 0.05666691101596056 2.534795626057801 >100 0.028332065884992843 4.124515869020867 >500 0.004722010962320522 2.317126280755099 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA 942 1.461416736479568 No Hit GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 549 0.8517173973750349 No Hit TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 477 0.7400167550963418 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 429 0.6655496602438797 No Hit GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA 375 0.5817741785348599 No Hit GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 237 0.36768128083403145 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.30717676626640605 No Hit ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 196 0.3040739706475534 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 177 0.27459741226845386 No Hit GACTAATATGCTTAAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGC 131 0.2032331130348444 No Hit GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 113 0.1753079524651711 No Hit GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 110 0.17065375903689223 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT 108 0.16755096341803966 No Hit GTATCAACGCAGAGTACGGGCGCGACCTCAGATCAGACGTGGCGACCCGCT 103 0.15979397437090817 No Hit GAGTACGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCA 92 0.14272859846721897 No Hit GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 88 0.1365230072295138 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT 85 0.1318688138012349 No Hit GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA 85 0.1318688138012349 No Hit GAACAAGAGAACACAACGAGCGACTGCCACAAAAAAAAGTGCACTCGGGAG 84 0.13031741599180863 No Hit GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA 83 0.12876601818238234 No Hit GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 80 0.12411182475410344 No Hit TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG 77 0.11945763132582457 No Hit TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA 75 0.11635483570697198 No Hit GACTAATATGCTTAAATTCAGCGGGTCGCCACGTCTGATCCTGTCTCTTAT 75 0.11635483570697198 No Hit GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 72 0.1117006422786931 No Hit GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC 71 0.1101492444692668 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.001551397809426293 13 0.0 0.0 0.001551397809426293 14 0.0 0.0 0.001551397809426293 15 0.0 0.0 0.001551397809426293 16 0.0 0.0 0.003102795618852586 17 0.0 0.0 0.003102795618852586 18 0.0 0.0 0.003102795618852586 19 0.0 0.0 0.003102795618852586 20 0.0 0.0 0.004654193428278879 21 0.0 0.0 0.01551397809426293 22 0.0 0.0 0.02016817152254181 23 0.0 0.0 0.03568214961680474 24 0.0 0.0 0.05429892332992026 25 0.0 0.0 0.07136429923360949 26 0.0 0.0 0.08532687951844611 27 0.0 0.0 0.13497160942008748 28 0.0 0.0 0.3506159049303422 29 0.0 0.0 1.132520400881194 30 0.0 0.0 1.968723820161966 31 0.0 0.0 2.719600359924292 32 0.0 0.0 3.4627199106394864 33 0.0 0.0 4.326848490489931 34 0.0 0.0 5.487294051940799 35 0.0 0.0 6.339011449315834 36 0.0 0.0 7.468429054578175 37 0.0 0.0 9.007415681529057 38 0.0 0.0 10.85202767693692 39 0.0 0.0 14.3085419963387 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTTTC 20 6.931659E-4 45.06838 1 GGTTTCT 30 0.0051125675 29.998966 2 CTCCGAG 40 6.103932E-4 28.124031 45 TGTAAGT 50 6.983158E-5 27.041025 1 ACTAATA 45 0.0012142775 24.999138 2 ACGGGCG 95 2.0387233E-8 23.683395 16 ATTAGTC 50 0.002242121 22.499224 34 GTAAGTA 60 2.4332029E-4 22.499224 2 AGCACTC 50 0.002242121 22.499224 12 TATTAGT 50 0.002242121 22.499224 33 CCCCCCT 50 0.002242121 22.499224 45 GGGCGCG 80 2.9040366E-6 22.499224 18 CATATTA 50 0.002242121 22.499224 31 ATATTAG 50 0.002242121 22.499224 32 CGGGCGC 85 4.913196E-6 21.17574 17 TCTCCGA 75 4.5178916E-5 20.999275 45 ACGGGGC 55 0.0038977547 20.45384 16 GCATATT 55 0.0038977547 20.45384 30 GGCGCGA 90 8.057115E-6 19.99931 19 GCGCGAC 90 8.057115E-6 19.99931 20 >>END_MODULE