FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_25.3410000000506e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_25.3410000000506e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64458
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA9421.461416736479568No Hit
GAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5490.8517173973750349No Hit
TATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4770.7400167550963418No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.6655496602438797No Hit
GGTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA3750.5817741785348599No Hit
GTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2370.36768128083403145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.30717676626640605No Hit
ATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1960.3040739706475534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.27459741226845386No Hit
GACTAATATGCTTAAATTCAGCGGGTCGCCACGTCTGATCTGAGGTCGCGC1310.2032331130348444No Hit
GTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA1130.1753079524651711No Hit
GTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.17065375903689223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT1080.16755096341803966No Hit
GTATCAACGCAGAGTACGGGCGCGACCTCAGATCAGACGTGGCGACCCGCT1030.15979397437090817No Hit
GAGTACGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCA920.14272859846721897No Hit
GAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA880.1365230072295138No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT850.1318688138012349No Hit
GTATCAACGCAGAGTACGGGGTGGTATCCTGTCTCTTATACACATCTCCGA850.1318688138012349No Hit
GAACAAGAGAACACAACGAGCGACTGCCACAAAAAAAAGTGCACTCGGGAG840.13031741599180863No Hit
GATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGA830.12876601818238234No Hit
GAGTACGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA800.12411182475410344No Hit
TGATACCACCCCGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCG770.11945763132582457No Hit
TTTTTGCGTTGATACCACCCCGTACTCTGCGTTGATACCCTGTCTCTTATA750.11635483570697198No Hit
GACTAATATGCTTAAATTCAGCGGGTCGCCACGTCTGATCCTGTCTCTTAT750.11635483570697198No Hit
GTATCAACGCAGAGTACGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA720.1117006422786931No Hit
GTATCAACGCAGAGTACGGGGTGGTATCAACGCAAAAACTGTCTCTTATAC710.1101492444692668No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTTC206.931659E-445.068381
GGTTTCT300.005112567529.9989662
CTCCGAG406.103932E-428.12403145
TGTAAGT506.983158E-527.0410251
ACTAATA450.001214277524.9991382
ACGGGCG952.0387233E-823.68339516
ATTAGTC500.00224212122.49922434
GTAAGTA602.4332029E-422.4992242
AGCACTC500.00224212122.49922412
TATTAGT500.00224212122.49922433
CCCCCCT500.00224212122.49922445
GGGCGCG802.9040366E-622.49922418
CATATTA500.00224212122.49922431
ATATTAG500.00224212122.49922432
CGGGCGC854.913196E-621.1757417
TCTCCGA754.5178916E-520.99927545
ACGGGGC550.003897754720.4538416
GCATATT550.003897754720.4538430
GGCGCGA908.057115E-619.9993119
GCGCGAC908.057115E-619.9993120
Produced by FastQC (version 0.11.2)