##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 18h_23.34100000005045.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8384 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.5994751908397 38.0 32.0 38.0 32.0 38.0 2 35.786617366412216 38.0 32.0 38.0 32.0 38.0 3 35.8275286259542 38.0 32.0 38.0 32.0 38.0 4 35.85663167938932 38.0 32.0 38.0 32.0 38.0 5 35.93070133587786 38.0 32.0 38.0 32.0 38.0 6 37.6012643129771 38.0 38.0 40.0 32.0 40.0 7 37.44119751908397 38.0 38.0 40.0 32.0 40.0 8 37.405057251908396 38.0 38.0 40.0 32.0 40.0 9 37.41865458015267 38.0 38.0 40.0 32.0 40.0 10 37.30450858778626 38.0 38.0 40.0 32.0 40.0 11 37.317867366412216 38.0 38.0 40.0 32.0 40.0 12 37.24117366412214 38.0 38.0 40.0 32.0 40.0 13 37.22674141221374 38.0 38.0 40.0 32.0 40.0 14 37.219942748091604 38.0 38.0 40.0 32.0 40.0 15 37.26550572519084 38.0 38.0 40.0 32.0 40.0 16 37.14396469465649 38.0 38.0 40.0 32.0 40.0 17 37.09983301526717 38.0 38.0 40.0 32.0 40.0 18 37.17175572519084 38.0 38.0 40.0 32.0 40.0 19 37.243082061068705 38.0 38.0 40.0 32.0 40.0 20 37.1950143129771 38.0 38.0 40.0 32.0 40.0 21 37.20825381679389 38.0 38.0 40.0 32.0 40.0 22 37.135138358778626 38.0 38.0 40.0 32.0 40.0 23 37.194417938931295 38.0 38.0 40.0 32.0 40.0 24 37.12261450381679 38.0 38.0 40.0 32.0 40.0 25 37.221374045801525 38.0 38.0 40.0 32.0 40.0 26 37.04937977099237 38.0 38.0 40.0 32.0 40.0 27 37.02278148854962 38.0 38.0 40.0 32.0 40.0 28 37.12690839694657 38.0 38.0 40.0 32.0 40.0 29 37.05570133587786 38.0 38.0 40.0 32.0 40.0 30 36.96600667938932 38.0 38.0 40.0 32.0 40.0 31 37.01312022900763 38.0 38.0 40.0 32.0 40.0 32 36.927242366412216 38.0 38.0 40.0 32.0 40.0 33 36.84852099236641 38.0 38.0 40.0 32.0 40.0 34 36.96886927480916 38.0 38.0 40.0 32.0 40.0 35 36.90064408396947 38.0 38.0 40.0 32.0 40.0 36 36.980796755725194 38.0 38.0 40.0 32.0 40.0 37 36.876073473282446 38.0 38.0 40.0 32.0 40.0 38 36.896588740458014 38.0 38.0 40.0 32.0 40.0 39 36.86319179389313 38.0 38.0 40.0 32.0 40.0 40 36.83468511450382 38.0 38.0 40.0 32.0 40.0 41 36.87738549618321 38.0 38.0 40.0 32.0 40.0 42 36.9537213740458 38.0 38.0 40.0 32.0 40.0 43 36.9161498091603 38.0 38.0 40.0 32.0 40.0 44 36.87225667938932 38.0 38.0 40.0 32.0 40.0 45 36.926884541984734 38.0 38.0 40.0 32.0 40.0 46 36.833611641221374 38.0 38.0 40.0 32.0 40.0 47 36.821207061068705 38.0 38.0 40.0 32.0 40.0 48 36.82526240458015 38.0 38.0 40.0 32.0 40.0 49 36.751192748091604 38.0 38.0 40.0 32.0 40.0 50 36.788167938931295 38.0 38.0 40.0 32.0 40.0 51 36.05403148854962 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 1.8070459077654775 2201 2 1.5171446426380584 2201 3 0.7805583499559532 2201 4 -0.43496459338358306 2201 5 0.12238834356432449 2201 6 1.30138947195897 2201 7 -0.2612036811872329 2201 8 1.2810394306899155 2201 9 1.4642261110599932 2201 10 2.0681637304581386 2201 11 1.9349512090157646 2201 12 1.96017126719223 2201 13 -1.272399708296092 2201 14 0.2870559607607248 2201 15 0.797263957944466 2201 16 -0.18382978384006066 2201 17 1.1757646547531309 2201 18 1.2638100660304659 2201 19 -1.0758600089603405 2201 20 -2.159813007715975 2201 21 0.6354354013584995 2201 22 -0.5740087831576233 2201 23 1.7429137371674415 2201 24 -0.054950008188086485 2201 25 1.55438210364197 2201 26 2.340928328798391 2201 27 1.6976989704374645 2201 28 0.8803718416999686 2201 29 1.249302753167882 2201 30 1.6808018872574735 2201 31 1.9773032203542016 2201 32 2.110472887857753 2201 33 -0.4598210896833379 2201 34 0.9706725223789405 2201 35 1.377065721422234 2201 36 1.4319262049628065 2201 37 1.876590076590091 2201 38 0.24180918793378936 2201 39 1.9422991379508616 2201 40 -1.1136296577600007 2201 41 1.4142421209547038 2201 42 1.5694267731993818 2201 43 1.4759943592591611 2201 44 1.0816116928575852 2201 45 1.2065214082792153 2201 46 1.9275390043071141 2201 47 -2.205600639081311 2201 48 0.7422387884867447 2201 49 0.15221266877311734 2201 50 -1.054250543179414 2201 51 2.23392102004356 1101 1 -0.5111359104163427 1101 2 -0.6192189937255748 1101 3 -2.537623468225867 1101 4 -1.29860095701995 1101 5 -2.324581353405378 1101 6 -0.6077014371319365 1101 7 1.0342508642673138 1101 8 0.34922124887173567 1101 9 1.0248321716660556 1101 10 -0.44698778469337697 1101 11 1.5107087847733354 1101 12 1.1571409641619326 1101 13 -0.7496724355688187 1101 14 -3.5993076756029083 1101 15 -1.8997057390252365 1101 16 0.9904126404023685 1101 17 0.2894010183894977 1101 18 1.294113096333497 1101 19 1.1968672637669329 1101 20 1.2795809316779625 1101 21 1.514223280146382 1101 22 -4.263402722551561 1101 23 -1.0904195961658942 1101 24 1.285959082721007 1101 25 -0.20319365393378774 1101 26 -1.9014959136258511 1101 27 -2.7719979992595043 1101 28 0.2591597204878511 1101 29 -0.4476669438018206 1101 30 -0.5313193248637376 1101 31 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49 2.2593555259159714 1208 50 2.4219399330110676 1208 51 -0.2779837418612061 1209 1 -0.09295409223452111 1209 2 0.8171446426380555 1209 3 -1.3194416500440482 1209 4 1.2650354066164198 1209 5 0.8390550102309859 1209 6 -2.0986105280410285 1209 7 -0.2445370145205672 1209 8 -1.868960569310083 1209 9 0.7642261110599975 1209 10 0.1348303971248015 1209 11 -1.3983821243175711 1209 12 -1.2731620661411043 1209 13 -0.9557330416294221 1209 14 1.3703892940940605 1209 15 -0.1360693753888711 1209 16 -0.43382978384006066 1209 17 -0.6075686785802006 1209 18 0.5304767326971316 1209 19 1.324139991039658 1209 20 1.2735203256173548 1209 21 1.5021020680251667 1209 22 0.475991216842381 1209 23 0.9095804038341058 1209 24 -0.27161667485475505 1209 25 -2.0456178963580243 1209 26 -1.1257383378682775 1209 27 -0.20230102956254115 1209 28 0.3803718416999686 1209 29 -0.35069724683211945 1209 30 1.9474685539241392 1209 31 1.2773032203542058 1209 32 1.4771395545244204 1209 33 1.6568455769833292 1209 34 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0.6812421371117949 1215 41 2.478344685057273 1215 42 1.2489139526865642 1215 43 0.23240461566941661 1215 44 0.8636629749088627 1215 45 -0.960145258387449 1215 46 -0.05964048287237489 1215 47 2.217476283995609 1215 48 1.7037772500252046 1215 49 1.1714434380038838 1215 50 2.048313559384688 1215 51 1.381356917479458 1212 1 -0.5350593553924199 1212 2 -2.246013252098784 1212 3 -0.8773363868861495 1212 4 1.1176669855637869 1212 5 -1.1714713055584838 1212 6 -4.856505264883133 1212 7 -0.35330894434513027 1212 8 -0.5742237272048243 1212 9 -0.5971773977119312 1212 10 -0.7388538134015121 1212 11 -1.503645282212311 1212 12 0.31982038999925066 1212 13 0.8723371338091681 1212 14 1.3528454344449372 1212 15 0.621825361453233 1212 16 -0.57418066103304 1212 17 -1.2847616610363417 1212 18 -0.7537337936186574 1212 19 0.660982096302817 1212 20 1.6384326063191068 1212 21 0.7056108399549927 1212 22 -0.5696228182453424 1212 23 -0.8798932803764217 1212 24 -0.015476323977559048 1212 25 0.7824522790805659 1212 26 -0.9397734255875747 1212 27 -0.3461606786853437 1212 28 -0.28629482496669567 1212 29 -0.9787674222707139 1212 30 0.4878194311171242 1212 31 -3.3998897621019353 1212 32 -3.161456936703651 1212 33 -1.9291193352973721 1212 34 0.7689181364140225 1212 35 0.07443414247486402 1212 36 1.4582419944364915 1212 37 0.043256743256755215 1212 38 0.5795284861793988 1212 39 1.907211418652615 1212 40 -1.30661211390035 1212 41 0.5633649279722519 1212 42 -0.7551346303093851 1212 43 1.1514329557503942 1212 44 -0.541195324686278 1212 45 0.03985474161255098 1212 46 0.5678898815000935 1212 47 -0.4950743232918384 1212 48 0.34750194638148457 1212 49 -2.492524173332143 1212 50 0.4369775269960243 1212 51 -0.32309652381609055 1213 1 0.6706822714018443 1213 2 0.38078100627442524 1213 3 0.09874016813777331 1213 4 -3.0258736842926766 1213 5 -1.142763171587191 1213 6 -1.2440650734955767 1213 7 0.03425086426731383 1213 8 0.34922124887173567 1213 9 -0.24789510106121782 1213 10 1.2802849425793497 1213 11 0.237981512046062 1213 12 0.7935046005255657 1213 13 -1.2951269810233654 1213 14 -0.14476222105745506 1213 15 -0.1724330117525028 1213 16 0.3540490040387283 1213 17 0.4712192002076776 1213 18 -0.7967959945755965 1213 19 0.9241399910396595 1213 20 -1.9022372501402174 1213 21 -1.2130494471263447 1213 22 -1.1724936316424674 1213 23 -0.3631468688931676 1213 24 -0.714040917278993 1213 25 -0.29410274484287413 1213 26 -0.8105868227167647 1213 27 -0.49927072653223803 1213 28 -2.6499311886030625 1213 29 1.1886966925618196 1213 30 1.195953402408989 1213 31 -3.007545264494283 1213 32 -0.41983014244527794 1213 33 -2.5128513927136353 1213 34 0.22824827995469832 1213 35 0.21797481233132743 1213 36 -0.9771647041281 1213 37 1.225074925074935 1213 38 1.3402940364186335 1213 39 2.366541562193291 1213 40 0.8560673119369682 1213 41 -1.0251518184392339 1213 42 -2.0517853480127357 1213 43 0.12750951077431694 1213 44 -0.29717618593029727 1213 45 -4.141963440205629 1213 46 1.2760238527919583 1213 47 2.0216720881914156 1213 48 2.1513296975776512 1213 49 2.493121759682211 1213 50 -0.8118263007551718 1213 51 -1.758503222380682 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 2.0 22 9.0 23 6.0 24 21.0 25 48.0 26 72.0 27 69.0 28 115.0 29 135.0 30 213.0 31 225.0 32 262.0 33 315.0 34 385.0 35 482.0 36 607.0 37 803.0 38 1454.0 39 3160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.70102600811262 24.60033404915295 15.151515151515152 27.54712479121928 2 12.692353572706669 25.22963139687463 42.13288798759394 19.945127042824765 3 15.660782442748092 28.554389312977097 35.48425572519084 20.30057251908397 4 11.891698473282442 22.984255725190838 39.93320610687023 25.190839694656486 5 11.712786259541986 38.60925572519084 37.85782442748092 11.820133587786259 6 24.785305343511453 44.48950381679389 19.27480916030534 11.450381679389313 7 20.96851145038168 34.00524809160305 25.62022900763359 19.40601145038168 8 19.8831106870229 41.74618320610687 22.96040076335878 15.410305343511451 9 22.16125954198473 18.76192748091603 25.441316793893133 33.63549618320611 10 14.360687022900764 32.478530534351144 34.25572519083969 18.905057251908396 11 28.11307251908397 26.371660305343514 29.46087786259542 16.0543893129771 12 17.748091603053435 28.614026717557252 31.88215648854962 21.755725190839694 13 23.1631679389313 24.37977099236641 31.92986641221374 20.527194656488547 14 17.270992366412212 24.499045801526716 29.80677480916031 28.423187022900763 15 20.3125 31.774809160305345 25.071564885496183 22.841125954198475 16 23.282442748091604 30.558206106870227 27.480916030534353 18.678435114503817 17 18.08206106870229 34.43463740458015 29.06727099236641 18.416030534351144 18 22.3043893129771 31.21421755725191 28.95992366412214 17.521469465648856 19 20.443702290076335 29.198473282442748 28.661736641221374 21.69608778625954 20 18.535305343511453 29.80677480916031 29.31774809160305 22.34017175572519 21 19.525286259541986 29.69942748091603 27.838740458015266 22.936545801526716 22 19.728053435114504 32.80057251908397 27.19465648854962 20.276717557251906 23 20.801526717557252 29.150763358778626 27.49284351145038 22.55486641221374 24 22.53101145038168 29.46087786259542 28.435114503816795 19.572996183206108 25 20.07395038167939 32.37118320610687 27.946087786259543 19.608778625954198 26 23.330152671755723 29.162690839694655 28.411259541984734 19.095896946564885 27 20.097805343511453 32.99141221374045 28.24427480916031 18.666507633587788 28 19.739980916030532 28.97185114503817 28.769083969465647 22.519083969465647 29 19.27480916030534 31.50047709923664 30.05725190839695 19.16746183206107 30 21.922709923664122 29.138835877862597 29.103053435114507 19.83540076335878 31 20.622614503816795 29.639790076335874 29.69942748091603 20.0381679389313 32 20.240935114503817 29.580152671755727 29.80677480916031 20.372137404580155 33 18.92891221374046 29.079198473282442 31.61975190839695 20.372137404580155 34 20.08587786259542 27.123091603053435 30.62977099236641 22.16125954198473 35 19.69227099236641 27.62404580152672 32.07299618320611 20.610687022900763 36 20.264790076335878 27.06345419847328 31.46469465648855 21.20706106870229 37 18.52337786259542 28.11307251908397 31.560114503816795 21.803435114503817 38 17.771946564885496 25.310114503816795 35.29341603053435 21.62452290076336 39 18.30868320610687 22.173187022900763 33.63549618320611 25.88263358778626 40 17.62881679389313 22.578721374045802 36.271469465648856 23.520992366412212 41 18.213263358778626 22.864980916030532 33.86211832061068 25.059637404580155 42 20.515267175572518 22.697996183206108 33.86211832061068 22.924618320610687 43 16.09017175572519 22.650286259541986 37.39265267175573 23.8668893129771 44 15.18368320610687 25.763358778625957 35.29341603053435 23.759541984732824 45 14.026717557251908 24.236641221374043 37.845896946564885 23.890744274809162 46 17.270992366412212 23.05582061068702 34.93559160305343 24.737595419847327 47 13.311068702290077 25.405534351145036 34.85209923664122 26.431297709923662 48 13.62118320610687 24.09351145038168 34.49427480916031 27.791030534351147 49 16.424141221374043 25.059637404580155 32.907919847328245 25.608301526717558 50 12.666984732824426 24.940362595419845 36.56965648854962 25.822996183206108 51 11.714183466539426 24.99105332219969 32.07682214004533 31.217941071215556 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 2.0 11 1.5 12 1.0 13 2.0 14 3.0 15 3.0 16 3.0 17 2.0 18 1.0 19 3.5 20 6.0 21 4.5 22 3.0 23 4.5 24 6.0 25 22.5 26 92.5 27 146.0 28 309.5 29 473.0 30 675.5 31 878.0 32 946.0 33 1014.0 34 980.0 35 946.0 36 840.0 37 734.0 38 673.5 39 613.0 40 550.0 41 487.0 42 446.5 43 406.0 44 389.5 45 373.0 46 375.0 47 377.0 48 371.0 49 365.0 50 406.5 51 448.0 52 334.5 53 221.0 54 189.5 55 158.0 56 146.0 57 134.0 58 121.5 59 109.0 60 97.5 61 86.0 62 83.0 63 80.0 64 73.5 65 67.0 66 60.0 67 53.0 68 52.5 69 52.0 70 45.0 71 38.0 72 31.0 73 24.0 74 21.0 75 13.0 76 8.0 77 9.0 78 10.0 79 5.5 80 1.0 81 0.5 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023854961832061067 2 0.011927480916030533 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.011927480916030533 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 8384.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.45419847328245 #Duplication Level Percentage of deduplicated Percentage of total 1 93.51402373247033 82.71708015267176 2 4.449838187702265 7.872137404580153 3 0.9573894282632146 2.5405534351145036 4 0.5663430420711975 2.0038167938931295 5 0.20226537216828477 0.89456106870229 6 0.16181229773462785 0.8587786259541985 7 0.013484358144552318 0.08349236641221373 8 0.013484358144552318 0.09541984732824427 9 0.0 0.0 >10 0.09439050701186624 1.2166030534351144 >50 0.026968716289104636 1.717557251908397 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC 93 1.1092557251908397 No Hit CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 51 0.6083015267175572 No Hit CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC 20 0.2385496183206107 RNA PCR Primer, Index 39 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC 16 0.19083969465648853 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT 16 0.19083969465648853 No Hit CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC 14 0.16698473282442747 No Hit CTCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCG 13 0.15505725190839695 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 12 0.1431297709923664 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 11 0.13120229007633588 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.011927480916030535 2 0.0 0.0 0.011927480916030535 3 0.0 0.0 0.011927480916030535 4 0.0 0.0 0.011927480916030535 5 0.0 0.0 0.011927480916030535 6 0.0 0.0 0.011927480916030535 7 0.0 0.0 0.011927480916030535 8 0.0 0.0 0.011927480916030535 9 0.0 0.0 0.011927480916030535 10 0.0 0.0 0.02385496183206107 11 0.0 0.0 0.0357824427480916 12 0.0 0.0 0.04770992366412214 13 0.0 0.0 0.04770992366412214 14 0.0 0.0 0.04770992366412214 15 0.0 0.0 0.04770992366412214 16 0.0 0.0 0.04770992366412214 17 0.0 0.0 0.04770992366412214 18 0.0 0.0 0.08349236641221373 19 0.0 0.0 0.08349236641221373 20 0.0 0.0 0.09541984732824428 21 0.0 0.0 0.09541984732824428 22 0.0 0.0 0.10734732824427481 23 0.0 0.0 0.13120229007633588 24 0.0 0.0 0.17891221374045801 25 0.0 0.0 0.17891221374045801 26 0.0 0.0 0.17891221374045801 27 0.0 0.0 0.26240458015267176 28 0.0 0.0 0.42938931297709926 29 0.0 0.0 0.8110687022900763 30 0.0 0.0 1.180820610687023 31 0.0 0.0 2.206583969465649 32 0.0 0.0 3.79293893129771 33 0.0 0.0 5.236164122137405 34 0.0 0.0 7.335400763358779 35 0.0 0.0 9.303435114503817 36 0.0 0.0 11.999045801526718 37 0.0 0.0 14.992843511450381 38 0.0 0.0 17.938931297709924 39 0.0 0.0 21.564885496183205 >>END_MODULE >>Kmer Content pass >>END_MODULE