FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_22.34100000005038.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_22.34100000005038.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055812
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTAT24750.23441673328206158No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATCC19780.18734395896239103No Hit
ATTCTAGAATTTGCAGTAGCATTAATTCAAGCCTACGTATTCACCCTCCTA17400.1648020670346615No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAA11290.10693191590927173No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCG301.13990856E-437.4997227
CAACGAC801.0004442E-1030.93727133
TAGCCCG406.1660755E-428.1247925
GGGACGA507.1582144E-526.99980236
TATAGGG1550.026.1288412
GTATCAA8550.026.0598451
ATCTCCG5750.025.82711845
TCGCCTA450.001226541424.99981510
AACGACT1001.3442332E-924.74981934
CGATTGC651.5236856E-524.2305936
AACCCGA2100.023.5712556
CCCGAAT2300.023.4780868
CATCTCC16850.023.36888745
GCGTTTA1650.023.18164835
GGGCGTT1750.023.14268733
GGTCCTA1850.023.1079398
GGTATCA3250.022.8524781
AAACCGA500.002264585622.4998366
GGGGTCC1800.022.4998346
GGGGACG602.467448E-422.49983235
Produced by FastQC (version 0.11.2)