FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_21.3410000000502b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_21.3410000000502b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299304
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC352.8211728E-432.1417058
TGGGCGA300.005148406629.99892220
TATGCCG856.4828782E-926.47065743
ATCTCCG12300.023.78054845
TGCCGTC952.1158485E-823.68427345
CCGCTCA500.002265240522.4991919
ATCGGAC500.002265240522.4991913
CATCTCC31350.020.45459745
ACGGAGT706.985828E-419.2850238
ACCGTGC706.985828E-419.2850239
GCCCACG1302.7730493E-819.0385118
CGAGTAA600.006512292718.74932710
CGTGCAA851.2049262E-418.52874611
AGCGCTT750.001110310218.00004612
AACGGAG750.00111059717.9993537
TTGCGGT2053.6379788E-1217.56169727
CGCCTAA901.8664254E-417.49937222
GCCTTAC800.001706577316.881541
GCGCTTA800.001710978316.87504413
AGTCGTA800.001710978316.87504417
Produced by FastQC (version 0.11.2)