FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_2.34100000004e8d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_2.34100000004e8d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330646
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTCGC207.027269E-444.99833729
GTGCCGA250.002105324835.9986711
GGTATCA3100.032.6749081
GTATTGA300.00513154630.0125061
ATATAAC300.00513154630.0125061
GTATCAA7750.029.0443631
CTGTACC801.04942956E-725.3115634
GTATAGT450.00122173325.0104241
ATAGTAG450.001224970724.9990753
CGTTACG1650.023.18096224
TATAGTA500.002261710622.4991682
CAGTACC602.4629716E-422.4991664
TGTAAGT1750.021.8662551
GTTACGG2750.021.2719425
GGTGCGA550.00393154120.453796
TATACAG550.00393154120.453795
TAACAGT807.544356E-519.6867716
TGGTGCG807.544356E-519.6867715
TTACACT1151.5873957E-719.56449334
TATCAAC11550.019.47982