FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_19.34100000005001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_19.34100000005001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376554
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTACG250.002104398636.0031924
GTATCAA4350.026.9203851
GGTATCA1850.025.5631711
GCTAAGT551.3576297E-424.5672021
CTAGACA651.5207392E-524.2296984
GATTTAC500.002250190622.5199361
ACGGGTA500.00226238622.49900415
TGCACCG500.00226238622.4990045
TATACTG754.593652E-520.999075
GGGGGGC754.593652E-520.9990719
TAGACAG654.2322057E-420.7683125
CTAGGAC908.230227E-619.9991153
GTATAAA807.494558E-519.7049451
TACTTAC807.548338E-519.68662831
TATCAAC5500.019.6381022
TAACCAT706.973619E-419.284866
GAGGCCG706.973619E-419.284865
AACGCAG5650.019.1141976
TCAACGC5650.019.1141974
CAACGCA5750.018.7817785