FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_18.34100000004ff6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_18.34100000004ff6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851337
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG401.5602327E-533.7495643
GGCGTGC450.001226386624.9996748
ACGCCTA551.3679167E-424.54513421
CGCCTAA551.3679167E-424.54513422
ATCTCCG4250.022.7644145
TAGCGTA500.002264302722.4997067
CCCACGA802.9735875E-622.49970619
GGGGTCC702.7022017E-522.4997066
GTAGACG1056.103983E-821.42829119
GGTATCA3700.021.2872561
CCGAGCC754.5992125E-521.0009614
TCGTACC754.601295E-520.99972526
TGCCGTC654.23736E-420.76895945
CATCTCC15300.020.58796745
ATTACGC550.00393199420.4578841
CGCACTC550.003936009520.4542795
TAGCCTA1703.6379788E-1219.8526825
AAGATCG807.560814E-519.6872445
CGAGCCC706.9792866E-419.28659415
ATAGCGT706.982046E-419.2854616
Produced by FastQC (version 0.11.2)