FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_15.34100000004fcf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_15.34100000004fcf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260590
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC11210.4301776737403585No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTAT5730.21988564411527686No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTAA3020.11589086304155953No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGC2860.10975094976783453No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1600.037.9682645
TATACCG250.002104257535.9995425
TATGCCG1700.035.7348443
AATCTCG1550.033.3866736
CTCGTAT1850.032.83741839
TCTCGTA1850.032.83741838
ATGCCGT1850.032.83741844
ACGAGAC1850.032.83741822
CCACGAG1850.032.83741820
GCCCACG1900.031.97327218
CGTATGC1900.031.97327241
CACGAGA1900.031.97327221
CCCACGA1900.031.97327219
CGAGACA1700.031.76430123
GAGCCCA2000.031.49959816
TCGTATG1950.031.1534540
CGAGCCC2000.030.37461315
GGTATCA901.2732926E-1130.0168971
ATCGTAG300.00514036329.99961713
ACCGTCC300.00514036329.9996178
Produced by FastQC (version 0.11.2)