FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_14.34100000004fb2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_14.34100000004fb2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757621
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCT9330.12314864556288699No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAG8160.1077055678234896No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1700.029.11743443
TGCCGTC1700.029.11743445
TACATCG406.164452E-428.1247945
CTCGTAT1900.027.2366439
CCCACGA2000.026.99980219
CGAGACA1500.026.99980223
AATCTCG1800.026.24980736
ACGAGAC2000.025.87481122
ATCTCGT2100.025.71409637
TCGTATG2000.024.74981940
TCTCGTA2100.024.64267538
GCCCACG2200.024.54527518
CACGAGA2150.024.0695921
CCACGAG2350.023.93599320
CGAGCCC2200.023.52255415
TCCGAGC2350.022.97855413
GTATCAA7850.022.075731
ATGCCGT2250.021.9998444
CCGAGCC2500.021.59984214
CTACGTA807.559079E-519.68735533
Produced by FastQC (version 0.11.2)