H23NVBCXX l02n01 18h_13.34100000004fa6.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 18h_13.34100000004fa6.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6784
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	121	1.7836084905660379	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGTC	70	1.0318396226415094	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC	69	1.0170990566037736	RNA PCR Primer, Index 45 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGTTATACATCTCGTATGCCGTC	61	0.8991745283018868	RNA PCR Primer, Index 39 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	59	0.8696933962264151	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC	39	0.5748820754716981	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGCATGATCTCGTATGCCGTC	32	0.4716981132075472	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGT	29	0.4274764150943396	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC	21	0.3095518867924528	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC	19	0.2800707547169811	No Hit
TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT	16	0.2358490566037736	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGT	11	0.16214622641509435	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	9	0.13266509433962265	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC	9	0.13266509433962265	RNA PCR Primer, Index 23 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCTTC	8	0.1179245283018868	RNA PCR Primer, Index 45 (95% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACTGATTACAATCTCGTATGCCGT	7	0.10318396226415094	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGACGA	20	6.5461686E-4	45.0	24
CGAGACG	30	1.8615447E-6	44.999996	23
TATCTCG	35	5.3765307E-6	38.571426	36
CTGATTA	30	1.02424114E-4	37.499996	28
GAGACTG	30	1.02424114E-4	37.499996	24
TGCCGTC	75	0.0	36.0	45
GTATCTC	25	0.0019624818	36.0	35
ATCTCGT	80	1.8189894E-12	33.75	37
ATGCCGT	80	1.8189894E-12	33.75	44
CGTATGC	80	1.8189894E-12	33.75	41
TCGTATG	80	1.8189894E-12	33.75	40
CGAGACT	35	2.536242E-4	32.142857	23
AGCCCAC	85	3.6379788E-12	31.764706	17
GTATGCC	85	3.6379788E-12	31.764706	42
GCCCACG	85	3.6379788E-12	31.764706	18
CCACGAG	85	3.6379788E-12	31.764706	20
TATGCCG	85	3.6379788E-12	31.764706	43
GAGCCCA	85	3.6379788E-12	31.764706	16
CACGAGA	85	3.6379788E-12	31.764706	21
CCCACGA	85	3.6379788E-12	31.764706	19