FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_12.34100000004f99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_12.34100000004f99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802931
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT1058.54925E-1125.71286244
TAATACG450.001226628524.9986154
GTATCAA11200.024.9170931
AATCTCG651.523661E-524.2294336
TATGCCG1152.6557245E-1023.47696143
CTCGTAT1252.910383E-1123.39870339
ACGAGAC1401.364242E-1020.89169922
TGCCGTC1301.2259989E-920.76808245
GGTATCA4900.020.6685581
TCGTATG1552.3646862E-1120.32145540
CACGAGA1452.1827873E-1020.17129721
AACGCAG13450.019.9072026
TCAACGC13600.019.5209774
TCTCGTA1503.43789E-1019.4989238
TATCAAC14200.019.4883582
ACGCAGA13700.019.3796967
CAACGCA13800.019.2392645
CGCTACT600.00651090818.7489622
CGAGACT600.00651090818.74896223
CGCAGAG14250.018.6317088
Produced by FastQC (version 0.11.2)