FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 18h_11.34100000004f8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 18h_11.34100000004f8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491338
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG507.15097E-526.99957343
ATAAGAC507.15097E-526.9995733
GTATCAA6200.025.4209331
GGTATCA2900.024.844891
ACGAGAC651.5216314E-524.23038522
GTGTAGG1003.606874E-822.5156841
CGTATGC602.4649844E-422.49964341
CTAGTTG600.00650590918.7497043
GTATTGT1102.2356908E-618.4219231
TACTACT901.8631603E-417.4997234
GATACAC800.001709143916.8747333
CTCGTAT800.001709143916.87473339
CACGAGA800.001709143916.87473321
TACGGAT800.001709143916.87473327
TCTCGTA800.001709143916.87473338
TCGTATG800.001709143916.87473340
TATCAAC9300.016.6932852
TCAACGC8950.016.5919174
CAACGCA9000.016.4997395
TATGGTG1401.2513283E-616.0711755