##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 18h_10.34100000004f7f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4031 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 34.80203423468122 38.0 32.0 38.0 32.0 38.0 2 35.08955594145373 38.0 32.0 38.0 32.0 38.0 3 35.06177127263706 38.0 32.0 38.0 32.0 38.0 4 35.32473331679484 38.0 32.0 38.0 32.0 38.0 5 35.29124286777475 38.0 32.0 38.0 32.0 38.0 6 36.968246092780944 38.0 38.0 40.0 32.0 40.0 7 36.65021086579013 38.0 38.0 40.0 32.0 40.0 8 36.64252046638551 38.0 38.0 40.0 32.0 40.0 9 36.74423220044654 38.0 38.0 40.0 32.0 40.0 10 36.55197221533118 38.0 38.0 40.0 32.0 40.0 11 36.56958571074175 38.0 38.0 40.0 32.0 40.0 12 36.48027784668817 38.0 38.0 40.0 32.0 40.0 13 36.588935747953364 38.0 38.0 40.0 32.0 40.0 14 36.483254775489954 38.0 38.0 40.0 32.0 40.0 15 36.39196229223518 38.0 38.0 40.0 32.0 40.0 16 36.439593153063754 38.0 38.0 40.0 32.0 40.0 17 36.19697345571819 38.0 38.0 40.0 27.0 40.0 18 36.30538327958323 38.0 38.0 40.0 32.0 40.0 19 36.30364673778219 38.0 38.0 40.0 32.0 40.0 20 36.28553708757132 38.0 38.0 40.0 32.0 40.0 21 36.29843711237906 38.0 38.0 40.0 32.0 40.0 22 36.40858347804515 38.0 38.0 40.0 32.0 40.0 23 36.328454477797074 38.0 38.0 40.0 32.0 40.0 24 36.405606549243366 38.0 38.0 40.0 32.0 40.0 25 36.30513520218308 38.0 38.0 40.0 32.0 40.0 26 36.17539072190524 38.0 38.0 40.0 27.0 40.0 27 36.06970974944183 38.0 38.0 40.0 27.0 40.0 28 36.05755395683453 38.0 38.0 40.0 27.0 40.0 29 36.18580997271149 38.0 38.0 40.0 27.0 40.0 30 36.11436368146862 38.0 38.0 40.0 27.0 40.0 31 36.12924832547755 38.0 38.0 40.0 27.0 40.0 32 36.033986603820395 38.0 38.0 40.0 27.0 40.0 33 36.044405854626646 38.0 38.0 40.0 27.0 40.0 34 35.97370379558422 38.0 38.0 40.0 27.0 40.0 35 35.94839990076904 38.0 38.0 40.0 27.0 40.0 36 35.91292483254775 38.0 38.0 40.0 27.0 40.0 37 35.98313073678988 38.0 38.0 40.0 27.0 40.0 38 35.89580749193748 38.0 38.0 40.0 27.0 40.0 39 35.914661374348796 38.0 38.0 40.0 27.0 40.0 40 35.84445547010667 38.0 38.0 40.0 27.0 40.0 41 35.790622674274374 38.0 38.0 40.0 27.0 40.0 42 35.95187298437112 38.0 38.0 40.0 27.0 40.0 43 35.985859588191516 38.0 38.0 40.0 27.0 40.0 44 35.86901513272141 38.0 38.0 40.0 27.0 40.0 45 36.154304142892585 38.0 38.0 40.0 27.0 40.0 46 35.92880178615728 38.0 38.0 40.0 27.0 40.0 47 35.88067477052841 38.0 38.0 40.0 27.0 40.0 48 35.815926569089555 38.0 38.0 40.0 27.0 40.0 49 35.766807243860086 38.0 38.0 40.0 27.0 40.0 50 35.76953609526172 38.0 38.0 40.0 27.0 40.0 51 35.06053088563632 38.0 32.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 2.4893787202381077 2201 2 -0.9383928571428655 2201 3 -1.1968563988095227 2201 4 -1.1906063988095212 2201 5 -0.19053199404761045 2201 6 1.6502976190476275 2201 7 -1.3682663690476105 2201 8 0.08655133928572667 2201 9 0.9947358630952507 2201 10 0.2816220238095326 2201 11 0.18065476190476915 2201 12 -0.06303943452380167 2201 13 0.9072544642857139 2201 14 1.6768043154761898 2201 15 2.0941406249999943 2201 16 -0.3386532738095198 2201 17 0.8354910714285708 2201 18 -4.036718750000006 2201 19 -0.3300037202380821 2201 20 0.606287202380976 2201 21 1.8960751488095227 2201 22 1.2652157738095227 2201 23 1.60182291666667 2201 24 -1.1908296130952465 2201 25 1.2622581845238088 2201 26 0.9227492559523895 2201 27 0.9457775297619122 2201 28 1.1609561011904859 2201 29 1.239992559523813 2201 30 2.128608630952378 2201 31 2.4837425595238045 2201 32 2.825148809523803 2201 33 2.529352678571435 2201 34 0.8579985119047535 2201 35 2.5016555059523853 2201 36 -0.8335565476190538 2201 37 2.4105654761904844 2201 38 2.0474144345238017 2201 39 2.222860863095235 2201 40 -0.7447172619047606 2201 41 1.146986607142857 2201 42 1.6849144345238116 2201 43 0.8553199404761855 2201 44 2.0880394345238145 2201 45 1.976078869047619 2201 46 1.0448288690476346 2201 47 1.0931919642857224 2201 48 3.282440476190466 2201 49 -2.199032738095241 2201 50 -0.7480654761904688 2201 51 1.7452008928571416 1101 1 2.4893787202381077 1101 2 -1.271726190476194 1101 3 0.9698102678571416 1101 4 0.6427269345238145 1101 5 0.8094680059523895 1101 6 1.9836309523809632 1101 7 1.7984002976190538 1101 8 -1.9134486607142733 1101 9 1.3280691964285865 1101 10 1.2816220238095326 1101 11 1.6806547619047691 1101 12 2.770293898809534 1101 13 2.573921130952378 1101 14 3.0101376488095255 1101 15 1.42747395833333 1101 16 2.3280133928571445 1101 17 3.1688244047619065 1101 18 2.9632812499999943 1101 19 2.836662946428582 1101 20 1.7729538690476403 1101 21 0.8960751488095227 1101 22 -0.06811755952381304 1101 23 -1.89817708333333 1101 24 2.8091703869047535 1101 25 2.262258184523809 1101 26 2.256082589285718 1101 27 2.945777529761912 1101 28 2.8276227678571573 1101 29 1.5733258928571487 1101 30 2.4619419642857068 1101 31 1.8170758928571402 1101 32 -1.3415178571428612 1101 33 2.8626860119047635 1101 34 3.191331845238089 1101 35 3.5016555059523853 1101 36 3.3331101190476105 1101 37 3.4105654761904844 1101 38 2.0474144345238017 1101 39 2.8895275297619065 1101 40 1.9219494047619037 1101 41 2.9803199404761926 1101 42 0.018247767857147323 1101 43 1.6886532738095141 1101 44 -1.7452938988095141 1101 45 0.6427455357142833 1101 46 1.3781622023809703 1101 47 2.759858630952394 1101 48 -3.884226190476202 1101 49 -1.865699404761905 1101 50 2.251934523809531 1101 51 3.411867559523813 2202 1 -3.6356212797618923 2202 2 -2.4800595238095298 2202 3 -1.2801897321428584 2202 4 -0.9822730654761855 2202 5 0.5594680059523895 2202 6 -0.01636904761903679 2202 7 -0.11826636904761045 2202 8 -2.121781994047609 2202 9 -1.7969308035714135 2202 10 -0.051711309523803095 2202 11 -0.27767857142856656 2202 12 -2.1880394345238017 2202 13 0.19892113095237818 2202 14 -2.32319568452381 2202 15 0.55247395833333 2202 16 -0.08865327380951982 2202 17 -3.7061755952380935 2202 18 1.8799479166666586 2202 19 -4.163337053571418 2202 20 -0.393712797619024 2202 21 -0.14559151785714164 2202 22 -1.3597842261904773 2202 23 1.22682291666667 2202 24 -1.2324962797619108 2202 25 -0.4460751488095269 2202 26 1.8394159226190538 2202 27 -1.970889136904752 2202 28 -3.2557105654761784 2202 29 -1.0516741071428513 2202 30 -1.8297247023809575 2202 31 -0.8079241071428598 2202 32 -4.424851190476197 2202 33 -1.4289806547619008 2202 34 -1.5170014880952465 2202 35 -5.20667782738095 2202 36 -1.2918898809523895 2202 37 -3.5477678571428513 2202 38 1.0474144345238017 2202 39 1.3061941964285708 2202 40 -2.1197172619047606 2202 41 0.8553199404761926 2202 42 3.2682477678571473 2202 43 2.02198660714285 2202 44 -4.07862723214285 2202 45 -1.273921130952381 2202 46 -1.3301711309523654 2202 47 1.176525297619058 2202 48 -1.842559523809534 2202 49 1.134300595238095 2202 50 1.5019345238095312 2202 51 0.20353422619047734 1102 1 1.4893787202381077 1102 2 1.8949404761904702 1102 3 -1.0301897321428584 1102 4 -1.3572730654761855 1102 5 -0.19053199404761045 1102 6 -2.8497023809523725 1102 7 -1.3682663690476105 1102 8 1.5865513392857267 1102 9 1.9947358630952507 1102 10 -0.051711309523803095 1102 11 1.6806547619047691 1102 12 1.770293898809534 1102 13 2.2405877976190425 1102 14 -0.1565290178571459 1102 15 0.42747395833333 1102 16 2.3280133928571445 1102 17 2.5021577380952422 1102 18 0.9632812499999943 1102 19 1.8366629464285822 1102 20 1.7729538690476403 1102 21 -2.6039248511904773 1102 22 -2.9014508928571416 1102 23 0.60182291666667 1102 24 2.142503720238089 1102 25 1.928924851190473 1102 26 1.9227492559523895 1102 27 -2.220889136904752 1102 28 1.8276227678571573 1102 29 0.9066592261904844 1102 30 0.2952752976190425 1102 31 -3.0162574404761955 1102 32 2.158482142857139 1102 33 2.196019345238099 1102 34 2.524665178571418 1102 35 2.834988839285714 1102 36 2.666443452380946 1102 37 1.743898809523813 1102 38 2.0474144345238017 1102 39 1.8895275297619065 1102 40 0.5886160714285751 1102 41 1.3136532738095283 1102 42 0.851581101190483 1102 43 1.3553199404761855 1102 44 1.0880394345238145 1102 45 1.6427455357142833 1102 46 1.711495535714299 1102 47 3.426525297619058 1102 48 2.9491071428571303 1102 49 1.9676339285714306 1102 50 1.5852678571428669 1102 51 3.7452008928571416 2203 1 0.4893787202381077 2203 2 -1.1050595238095298 2203 3 0.9698102678571416 2203 4 0.6427269345238145 2203 5 -3.3571986607142783 2203 6 0.1502976190476275 2203 7 -3.3682663690476105 2203 8 1.9198846726190553 2203 9 1.9947358630952507 2203 10 1.948288690476197 2203 11 2.3473214285714334 2203 12 1.1036272321428626 2203 13 2.573921130952378 2203 14 2.0101376488095255 2203 15 1.7608072916666657 2203 16 -1.5053199404761841 2203 17 2.1688244047619065 2203 18 -6.036718750000009 2203 19 1.8366629464285822 2203 20 1.7729538690476403 2203 21 1.8960751488095227 2203 22 0.5985491071428584 2203 23 -2.89817708333333 2203 24 0.8091703869047535 2203 25 2.928924851190473 2203 26 0.42274925595238955 2203 27 -6.720889136904752 2203 28 0.16095610119048587 2203 29 -2.9266741071428513 2203 30 -6.2047247023809575 2203 31 -3.0162574404761955 2203 32 2.4918154761904674 2203 33 2.196019345238099 2203 34 2.524665178571418 2203 35 2.834988839285714 2203 36 0.6664434523809462 2203 37 2.743898809523813 2203 38 2.714081101190466 2203 39 2.8895275297619065 2203 40 2.4219494047619037 2203 41 2.9803199404761926 2203 42 2.1849144345238116 2203 43 3.02198660714285 2203 44 3.0880394345238145 2203 45 1.976078869047619 2203 46 1.3781622023809703 2203 47 1.7598586309523938 2203 48 -2.8842261904761983 2203 49 2.300967261904759 2203 50 2.585267857142867 2203 51 4.078534226190477 1103 1 0.7750930059523924 1103 2 0.32351190476190084 1103 3 2.1126674107142875 1103 4 0.07129836309524507 1103 5 0.23803943452382015 1103 6 1.8407738095238173 1103 7 1.9888764880952436 1103 8 0.6817894345238216 1103 9 1.0423549107143018 1103 10 -0.194568452380949 1103 11 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1.2836681547619122 1103 48 -2.4318452380952493 1103 49 1.9200148809523796 1103 50 2.8233630952381006 1103 51 0.07853422619047734 2204 1 0.4893787202381077 2204 2 0.8949404761904702 2204 3 0.9698102678571416 2204 4 0.6427269345238145 2204 5 0.8094680059523895 2204 6 1.316964285714299 2204 7 1.1317336309523895 2204 8 0.9198846726190553 2204 9 -0.17193080357141355 2204 10 2.2816220238095326 2204 11 0.6806547619047691 2204 12 1.4369605654761983 2204 13 1.5739211309523782 2204 14 3.0101376488095255 2204 15 1.42747395833333 2204 16 -1.8386532738095198 2204 17 1.6688244047619065 2204 18 2.9632812499999943 2204 19 -0.9966703869047535 2204 20 2.4396205357143117 2204 21 1.562741815476194 2204 22 2.2652157738095227 2204 23 -2.5648437499999943 2204 24 3.475837053571425 2204 25 2.262258184523809 2204 26 -2.5772507440476105 2204 27 3.279110863095248 2204 28 -2.339043898809514 2204 29 2.239992559523813 2204 30 2.4619419642857068 2204 31 2.81707589285714 2204 32 2.4918154761904674 2204 33 2.196019345238099 2204 34 2.8579985119047535 2204 35 3.1683221726190496 2204 36 3.3331101190476105 2204 37 2.0772321428571487 2204 38 1.0474144345238017 2204 39 2.556194196428571 2204 40 3.4219494047619037 2204 41 1.9803199404761926 2204 42 3.851581101190483 2204 43 2.02198660714285 2204 44 0.9213727678571502 2204 45 1.6427455357142833 2204 46 -1.1218377976190297 2204 47 2.759858630952394 2204 48 3.6157738095238017 2204 49 3.634300595238095 2204 50 2.251934523809531 2204 51 -0.7547991071428584 2205 1 0.4893787202381077 2205 2 -0.10505952380952976 2205 3 0.9698102678571416 2205 4 0.6427269345238145 2205 5 1.8094680059523895 2205 6 1.9836309523809632 2205 7 1.7984002976190538 2205 8 1.9198846726190553 2205 9 -1.8385974702380778 2205 10 0.6149553571428612 2205 11 2.0139880952380977 2205 12 1.770293898809534 2205 13 1.5739211309523782 2205 14 1.6768043154761898 2205 15 0.7608072916666657 2205 16 1.3280133928571445 2205 17 2.1688244047619065 2205 18 2.6299479166666586 2205 19 2.169996279761918 2205 20 2.4396205357143117 2205 21 -0.27059151785714164 2205 22 1.931882440476187 2205 23 0.9351562500000057 2205 24 1.1425037202380892 2205 25 -3.237741815476191 2205 26 -0.9105840773809462 2205 27 1.6124441964285836 2205 28 0.8276227678571573 2205 29 -1.5933407738095156 2205 30 2.7952752976190425 2205 31 2.81707589285714 2205 32 2.4918154761904674 2205 33 1.0293526785714349 2205 34 -2.308668154761911 2205 35 0.6683221726190496 2205 36 -1.8335565476190538 2205 37 -0.08943452380951555 2205 38 2.3807477678571374 2205 39 1.8895275297619065 2205 40 1.088616071428575 2205 41 -0.01968005952380736 2205 42 -2.3150855654761884 2205 43 -1.8113467261904859 2205 44 1.4213727678571502 2205 45 1.6427455357142833 2205 46 3.0448288690476346 2205 47 2.0931919642857224 2205 48 3.6157738095238017 2205 49 1.634300595238095 2205 50 2.9186011904761955 2205 51 0.5785342261904773 1216 1 0.2393787202381077 1216 2 -2.1050595238095298 1216 3 -0.6551897321428584 1216 4 -0.9822730654761855 1216 5 0.5594680059523895 1216 6 1.2336309523809632 1216 7 2.1317336309523895 1216 8 1.753218005952391 1216 9 1.0780691964285865 1216 10 1.948288690476197 1216 11 2.0973214285714334 1216 12 1.9369605654761983 1216 13 1.0739211309523782 1216 14 1.1768043154761898 1216 15 0.67747395833333 1216 16 0.16134672619048018 1216 17 0.2938244047619065 1216 18 -1.4950520833333414 1216 19 -1.0383370535714178 1216 20 -1.643712797619024 1216 21 0.9794084821428584 1216 22 -1.3597842261904773 1216 23 1.60182291666667 1216 24 0.3925037202380892 1216 25 -0.6960751488095269 1216 26 -0.7855840773809462 1216 27 0.6541108630952479 1216 28 0.7442894345238216 1216 29 1.3233258928571487 1216 30 2.5452752976190425 1216 31 2.56707589285714 1216 32 1.325148809523803 1216 33 0.8210193452380992 1216 34 -2.0170014880952465 1216 35 1.9183221726190496 1216 36 2.0831101190476105 1216 37 2.5772321428571487 1216 38 -1.8275855654761983 1216 39 -1.1938058035714292 1216 40 2.5052827380952394 1216 41 1.7303199404761926 1216 42 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2.4080915178571516 1211 20 -0.036569940476169904 1211 21 1.2294084821428584 1211 22 2.4080729166666686 1211 23 2.744680059523816 1211 24 2.142503720238089 1211 25 1.3574962797619037 1211 26 2.1608444940476232 1211 27 2.279110863095248 1211 28 0.35143229166667567 1211 29 1.0018973214285793 1211 30 -2.0618675595238116 1211 31 -1.7543526785714292 1211 32 -3.889136904761912 1211 33 -0.8039806547619008 1211 34 -1.1420014880952465 1211 35 0.02546502976190368 1211 36 -0.6668898809523895 1211 37 1.0772321428571487 1211 38 -2.6668712797619136 1211 39 -2.0152343749999986 1211 40 -1.1018601190476147 1211 41 2.551748511904762 1211 42 1.946819196428578 1211 43 0.021986607142849834 1211 44 -2.1500558035714192 1211 45 0.5475074404761884 1211 46 1.1876860119047805 1211 47 0.4265252976190581 1211 48 -3.1461309523809646 1211 49 -5.07998511904762 1211 50 -0.6052083333333229 1211 51 -4.635751488095238 1208 1 -0.08204985119046171 1208 2 1.180654761904755 1208 3 1.2555245535714263 1208 4 0.9284412202380992 1208 5 -0.619103422619041 1208 6 -0.4449404761904674 1208 7 -3.8682663690476105 1208 8 -1.746781994047609 1208 9 -0.8147879464285595 1208 10 1.2340029761904816 1208 11 1.6330357142857181 1208 12 -2.134468005952371 1208 13 -6.140364583333337 1208 14 -0.4660528273809561 1208 15 -2.1439546130952394 1208 16 1.0899181547619108 1208 17 -3.402604166666663 1208 18 -1.0843377976190567 1208 19 -2.877622767857133 1208 20 -1.893712797619024 1208 21 1.8008370535714278 1208 22 0.6937872023809533 1208 23 1.0303943452381006 1208 24 0.4282180059523739 1208 25 -1.071075148809527 1208 26 -4.410584077380946 1208 27 -0.1494605654761827 1208 28 0.06571800595239097 1208 29 -0.42667410714285126 1208 30 -1.776153273809527 1208 31 2.102790178571425 1208 32 -2.317708333333343 1208 33 -5.518266369047616 1208 34 -5.427715773809531 1208 35 -3.8316778273809504 1208 36 -4.524032738095247 1208 37 -2.7799107142857054 1208 38 -2.0954427083333442 1208 39 -1.8723772321428598 1208 40 -0.81614583333333 1208 41 -2.1625372023809533 1208 42 -0.7674665178571374 1208 43 -4.263727678571435 1208 44 -6.8643415178571345 1208 45 -4.452492559523808 1208 46 0.616257440476204 1208 47 -0.8591889880952266 1208 48 -4.003273809523819 1208 49 -2.9371279761904745 1208 50 -2.0337797619047535 1208 51 -0.7786086309523768 1209 1 -1.939192708333323 1209 2 -2.3907738095238145 1209 3 -0.17304687500000426 1209 4 0.9284412202380992 1209 5 -1.619103422619041 1209 6 -2.587797619047606 1209 7 1.4174479166666742 1209 8 -0.03249627976189373 1209 9 1.6137834821428712 1209 10 0.3768601190476275 1209 11 -1.9383928571428513 1209 12 2.2941034226190524 1209 13 0.7167782738095241 1209 14 0.3910900297619051 1209 15 0.14175967261904532 1209 16 1.9470610119047649 1209 17 -0.2597470238095241 1209 18 1.201376488095228 1209 19 2.122377232142867 1209 20 -1.3222842261904546 1209 21 0.657979910714289 1209 22 2.1223586309523768 1209 23 1.0303943452381006 1209 24 1.5710751488095198 1209 25 2.786067708333327 1209 26 1.8751302083333385 1209 27 2.8505394345238173 1209 28 2.6371465773809675 1209 29 -1.1409598214285666 1209 30 -1.4904389880952422 1209 31 0.9599330357142861 1209 32 2.968005952380949 1209 33 -4.0896949404761855 1209 34 1.8579985119047535 1209 35 3.3111793154761955 1209 36 2.618824404761895 1209 37 -1.9227678571428513 1209 38 1.618843005952371 1209 39 3.12762276785714 1209 40 1.8981398809523853 1209 41 3.1231770833333385 1209 42 1.6611049107142932 1209 43 2.02198660714285 1209 44 2.1356584821428655 1209 45 2.5475074404761884 1209 46 -0.09802827380951129 1209 47 1.1408110119047734 1209 48 -1.86041666666668 1209 49 2.9200148809523796 1209 50 -1.0337797619047535 1209 51 2.364248511904762 1214 1 -1.5106212797618923 1214 2 -0.10505952380952976 1214 3 0.9698102678571416 1214 4 -1.3572730654761855 1214 5 0.9761346726190538 1214 6 0.6502976190476275 1214 7 -0.20159970238094616 1214 8 -0.08011532738094473 1214 9 1.9947358630952507 1214 10 -0.3850446428571388 1214 11 2.0139880952380977 1214 12 1.1036272321428626 1214 13 1.5739211309523782 1214 14 -2.82319568452381 1214 15 -1.2391927083333343 1214 16 -0.8386532738095198 1214 17 0.5021577380952422 1214 18 0.1299479166666586 1214 19 1.169996279761918 1214 20 -0.393712797619024 1214 21 2.2294084821428584 1214 22 2.2652157738095227 1214 23 2.2684895833333343 1214 24 0.6425037202380892 1214 25 1.5955915178571374 1214 26 1.2560825892857181 1214 27 1.9457775297619122 1214 28 0.16095610119048587 1214 29 -0.09334077380951555 1214 30 1.4619419642857068 1214 31 2.150409226190476 1214 32 0.1584821428571388 1214 33 -2.6373139880952365 1214 34 0.3579985119047535 1214 35 3.1683221726190496 1214 36 -1.1668898809523895 1214 37 2.743898809523813 1214 38 3.714081101190466 1214 39 1.2228608630952351 1214 40 -2.0780505952380963 1214 41 0.48031994047619264 1214 42 -0.9817522321428527 1214 43 1.3553199404761855 1214 44 0.9213727678571502 1214 45 1.6427455357142833 1214 46 2.711495535714299 1214 47 3.426525297619058 1214 48 2.282440476190466 1214 49 2.9676339285714306 1214 50 1.5852678571428669 1214 51 -1.7547991071428584 1215 1 0.4893787202381077 1215 2 2.8949404761904702 1215 3 0.9698102678571416 1215 4 0.6427269345238145 1215 5 0.8094680059523895 1215 6 0.6502976190476275 1215 7 -3.3682663690476105 1215 8 1.253218005952391 1215 9 -6.005264136904746 1215 10 0.6149553571428612 1215 11 -1.1526785714285666 1215 12 -2.0630394345238017 1215 13 1.5739211309523782 1215 14 2.67680431547619 1215 15 0.42747395833333 1215 16 0.6613467261904802 1215 17 3.1688244047619065 1215 18 1.296614583333323 1215 19 -0.3300037202380821 1215 20 0.7729538690476403 1215 21 0.8960751488095227 1215 22 0.5985491071428584 1215 23 1.9351562500000057 1215 24 -2.357496279761911 1215 25 -1.571075148809527 1215 26 -2.5772507440476105 1215 27 -2.220889136904752 1215 28 -2.6723772321428427 1215 29 1.239992559523813 1215 30 -7.7047247023809575 1215 31 -1.1829241071428598 1215 32 2.4918154761904674 1215 33 1.8626860119047635 1215 34 2.524665178571418 1215 35 -1.6650111607142861 1215 36 -1.1668898809523895 1215 37 2.743898809523813 1215 38 0.3807477678571374 1215 39 -2.443805803571429 1215 40 3.4219494047619037 1215 41 0.6469866071428569 1215 42 -3.815085565476185 1215 43 -0.31134672619048587 1215 44 -0.41196056547618554 1215 45 -0.523921130952381 1215 46 2.3781622023809703 1215 47 -2.4068080357142776 1215 48 0.9491071428571303 1215 49 -1.865699404761905 1215 50 1.5852678571428669 1215 51 -4.5881324404761905 1212 1 0.08937872023810911 1212 2 1.6949404761904674 1212 3 -1.8301897321428555 1212 4 0.24272693452381588 1212 5 1.6094680059523867 1212 6 -0.6163690476190382 1212 7 -3.0682663690476133 1212 8 0.8532180059523924 1212 9 0.5280691964285893 1212 10 -2.8517113095238003 1212 11 -2.8526785714285694 1212 12 0.23696056547619548 1212 13 1.1739211309523796 1212 14 -2.9231956845238116 1212 15 -4.372526041666667 1212 16 2.0613467261904788 1212 17 2.9688244047619037 1212 18 2.4299479166666558 1212 19 1.8366629464285822 1212 20 2.306287202380979 1212 21 1.629408482142857 1212 22 0.8652157738095241 1212 23 -2.598177083333333 1212 24 -0.05749627976191363 1212 25 1.928924851190473 1212 26 2.7894159226190567 1212 27 2.6791108630952465 1212 28 2.6942894345238244 1212 29 -0.026674107142852677 1212 30 1.395275297619044 1212 31 0.01707589285714306 1212 32 -2.7748511904761983 1212 33 2.196019345238099 1212 34 2.6579985119047507 1212 35 2.1683221726190496 1212 36 3.5331101190476133 1212 37 0.07723214285714874 1212 38 2.4474144345238003 1212 39 2.1561941964285722 1212 40 1.5552827380952365 1212 41 2.380319940476191 1212 42 2.918247767857146 1212 43 1.6219866071428513 1212 44 1.0213727678571516 1212 45 0.976078869047619 1212 46 3.2448288690476375 1212 47 2.8265252976190567 1212 48 3.082440476190463 1212 49 3.434300595238092 1212 50 1.8519345238095326 1212 51 -1.1214657738095255 1213 1 -2.1106212797618937 1213 2 1.6949404761904674 1213 3 -7.030189732142858 1213 4 1.4427269345238116 1213 5 1.6094680059523867 1213 6 1.3836309523809618 1213 7 0.731733630952391 1213 8 0.4532180059523938 1213 9 -4.471930803571411 1213 10 -3.251711309523806 1213 11 -2.8526785714285694 1213 12 -4.363039434523799 1213 13 -2.6260788690476247 1213 14 -2.32319568452381 1213 15 0.6274739583333329 1213 16 -2.5386532738095227 1213 17 -3.6311755952380906 1213 18 -1.3700520833333414 1213 19 -3.3633370535714207 1213 20 0.30628720238097884 1213 21 -5.770591517857142 1213 22 -6.734784226190477 1213 23 -4.198177083333327 1213 24 -4.857496279761911 1213 25 -6.271075148809526 1213 26 -4.210584077380943 1213 27 -3.5208891369047493 1213 28 -4.305710565476176 1213 29 -4.82667410714285 1213 30 -2.4047247023809604 1213 31 -3.982924107142857 1213 32 -6.374851190476196 1213 33 -4.803980654761901 1213 34 0.057998511904756356 1213 35 2.568322172619048 1213 36 -4.066889880952388 1213 37 -4.522767857142853 1213 38 -5.5525855654762 1213 39 -3.2438058035714263 1213 40 -5.244717261904761 1213 41 -2.619680059523809 1213 42 -2.081752232142854 1213 43 -4.778013392857151 1213 44 -0.5786272321428498 1213 45 -3.223921130952384 1213 46 -4.755171130952366 1213 47 -7.173474702380943 1213 48 -1.3175595238095354 1213 49 0.03430059523809348 1213 50 0.8519345238095326 1213 51 -2.72146577380952 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 3.0 23 11.0 24 11.0 25 36.0 26 49.0 27 64.0 28 69.0 29 112.0 30 136.0 31 159.0 32 160.0 33 202.0 34 242.0 35 258.0 36 323.0 37 456.0 38 721.0 39 1018.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.5169933019102 17.14214835028529 11.85809972711486 34.48275862068966 2 13.718680228231209 20.56561647233937 40.68469362441082 25.031009675018606 3 16.869263210121556 23.71619945422972 32.12602331927562 27.28851401637311 4 14.661374348796825 20.41677003225006 33.73852642024311 31.18332919871 5 13.991565368394939 35.996030761597616 37.11237906226742 12.900024807740015 6 29.843711237906227 39.518729843711235 16.100223269660134 14.537335648722403 7 24.336392954601834 32.89506325973704 21.11138675266683 21.657157032994295 8 22.500620193500374 40.114115604068466 20.367154552220292 17.018109650210867 9 24.609278094765568 16.819647730091788 18.2584966509551 40.312577524187546 10 15.38079880922848 33.6144877201687 30.09178863805507 20.912924832547755 11 32.547754899528655 23.12081369387249 26.14735797568841 18.184073430910445 12 21.08657901265195 25.700818655420495 29.198709997519224 24.013892334408336 13 27.28851401637311 20.962540312577524 29.32274869759365 22.426196973455717 14 19.374844951624905 21.979657653187797 26.12255023567353 32.52294715951377 15 23.319275613991568 28.776978417266186 22.029273133217565 25.87447283552468 16 24.85735549491441 27.486975936492186 24.83254775489953 22.823120813693873 17 20.937732572562638 31.95236913917142 26.370627635822373 20.73927065244356 18 25.750434135450263 26.59389729595634 26.14735797568841 21.508310592904987 19 23.220044653932025 26.271396675762837 25.30389481518234 25.2046638551228 20 22.153311833291987 27.611014636566612 23.741007194244602 26.4946663358968 21 22.054080873232447 26.71793599603076 25.22947159513768 25.998511535599107 22 23.220044653932025 30.141404118084843 24.237161994542298 22.40138923344083 23 21.58273381294964 26.370627635822373 26.395435375837263 25.651203175390723 24 25.775241875465145 26.59389729595634 25.57677995534607 22.054080873232447 25 22.178119573306873 31.332175638799303 25.179856115107913 21.309848672785908 26 25.948896055569335 26.74274373604565 25.60158769536095 21.706772513024063 27 21.905234433143143 30.46390473827834 25.725626395435373 21.905234433143143 28 21.45869511287522 26.246588935747955 26.618705035971225 25.676010915405605 29 23.865045894319028 28.25601587695361 26.12255023567353 21.75638799305383 30 25.00620193500372 25.47754899528653 26.618705035971225 22.897544033738527 31 23.443314314065987 25.998511535599107 27.73505333664103 22.823120813693873 32 21.979657653187797 27.4621681964773 26.569089555941456 23.98908459439345 33 22.624658893574797 26.8171669560903 27.66063011659638 22.897544033738527 34 23.170429173902257 24.485239394691146 29.397171917638303 22.947159513768295 35 21.880426693128253 24.58447035475068 30.364673778218805 23.170429173902257 36 24.237161994542298 24.063507814438104 27.214090796328456 24.485239394691146 37 22.47581245348549 26.4946663358968 27.635822376581494 23.39369883403622 38 21.657157032994295 22.252542793351527 32.696601339617956 23.39369883403622 39 20.5160009923096 22.575043413545025 28.95063259737038 27.958322996774992 40 21.185809972711485 20.764078392458448 33.51525676010915 24.534854874720914 41 19.49888365169933 22.27735053336641 30.19101959811461 28.03274621681965 42 21.657157032994295 21.557926072934755 32.423716199454226 24.36120069461672 43 19.151575291490946 23.51773753411064 32.622178119573306 24.708509054825107 44 17.51426445050856 26.643512775986107 32.225254279335154 23.61696849417018 45 17.6134954105681 23.195236913917142 33.837757380302655 25.353510295212107 46 21.4835028528901 22.59985115355991 30.538327958323 25.378318035226993 47 16.000992309600594 24.708509054825107 30.488712478293227 28.80178615728107 48 16.199454229719674 24.286777474572066 30.81121309848673 28.702555197221535 49 20.24311585214587 23.468122054080872 30.76159761845696 25.5271644753163 50 15.405606549243364 24.559662614735796 34.631605060778966 25.403125775241875 51 15.480029769288016 23.220044653932025 29.198709997519224 32.101215579260725 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.0 12 0.0 13 1.5 14 3.0 15 2.0 16 1.0 17 1.0 18 1.0 19 1.5 20 2.0 21 2.0 22 2.0 23 2.0 24 2.0 25 2.5 26 7.0 27 11.0 28 17.5 29 24.0 30 36.5 31 49.0 32 69.0 33 89.0 34 123.5 35 158.0 36 211.0 37 264.0 38 301.0 39 338.0 40 378.0 41 418.0 42 416.0 43 414.0 44 409.5 45 405.0 46 392.5 47 380.0 48 345.0 49 310.0 50 285.5 51 261.0 52 249.0 53 237.0 54 201.0 55 165.0 56 137.0 57 109.0 58 104.0 59 99.0 60 86.5 61 74.0 62 67.0 63 60.0 64 56.0 65 52.0 66 46.5 67 41.0 68 32.0 69 23.0 70 17.5 71 12.0 72 8.5 73 5.0 74 6.0 75 8.0 76 9.0 77 5.5 78 2.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 4031.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.03076159761845 #Duplication Level Percentage of deduplicated Percentage of total 1 99.17334022216482 95.23691391714215 2 0.49082924308964093 0.9426941205656165 3 0.07749935417204858 0.2232696601339618 4 0.07749935417204858 0.2976928801786157 5 0.0 0.0 6 0.0 0.0 7 0.025833118057349523 0.1736541801041925 8 0.025833118057349523 0.19846192011907715 9 0.0 0.0 >10 0.12916559028674762 2.927313321756388 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTC 47 1.1659637806995784 RNA PCR Primer, Index 45 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACAGGATGTGATCTCGTATGCCGTC 37 0.9178863805507318 No Hit CTTATACACATCTCCGAGCCCACGAGACGAGAGTTGATCTCGTATGCCGTC 14 0.347308360208385 RNA PCR Primer, Index 23 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 10 0.24807740014884647 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC 10 0.24807740014884647 No Hit CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC 8 0.19846192011907715 No Hit TTATACACATCTCCGAGCCCACGAGACGTTCATTAATCTCGTATGCCGTCT 7 0.1736541801041925 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.024807740014884644 10 0.0 0.0 0.024807740014884644 11 0.0 0.0 0.024807740014884644 12 0.0 0.0 0.024807740014884644 13 0.0 0.0 0.024807740014884644 14 0.0 0.0 0.024807740014884644 15 0.0 0.0 0.024807740014884644 16 0.0 0.0 0.024807740014884644 17 0.0 0.0 0.024807740014884644 18 0.0 0.0 0.024807740014884644 19 0.0 0.0 0.024807740014884644 20 0.0 0.0 0.024807740014884644 21 0.0 0.0 0.024807740014884644 22 0.0 0.0 0.024807740014884644 23 0.0 0.0 0.04961548002976929 24 0.0 0.0 0.04961548002976929 25 0.0 0.0 0.04961548002976929 26 0.0 0.0 0.04961548002976929 27 0.0 0.0 0.14884644008930786 28 0.0 0.0 0.24807740014884644 29 0.0 0.0 0.47134706028280826 30 0.0 0.0 0.8930786405358472 31 0.0 0.0 1.8357727611014636 32 0.0 0.0 2.852890101711734 33 0.0 0.0 4.068469362441082 34 0.0 0.0 5.829818903497891 35 0.0 0.0 7.343091044405854 36 0.0 0.0 8.831555445298934 37 0.0 0.0 10.865790126519475 38 0.0 0.0 13.123294467873977 39 0.0 0.0 15.65368394939221 >>END_MODULE >>Kmer Content pass >>END_MODULE