FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_96.3410000000749a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_96.3410000000749a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC55.617977528089887No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC22.247191011235955No Hit
CACCACCACTGCCTGGCTTTAAGTGAGATTTAAGACTCATATGAAGCCACC11.1235955056179776No Hit
TCCTGTGCGCGCCCGACCTAGACCCCTCGGGTGTCCCAGGCGCTGTCTCTT11.1235955056179776No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC11.1235955056179776No Hit
TCTCTGCAGGCCGCCTGGGAACCAGTGTCAGGAAGACCCTGCTGCTCTGCT11.1235955056179776No Hit
TCTTAAAGGAGCCTTCTGTCTTTAACAAAAAACAAACAGATACTTTGGTTA11.1235955056179776No Hit
TGCAGGCAAAGAAGAAGGAGCTTGCGAGGAGAGATGACATTGAGGACGGAG11.1235955056179776No Hit
ATAAAAATGAGTGCAATAATTGCCAAAAAGCTGTAGATCCAGATTGACTGA11.1235955056179776No Hit
ACCCAACCTAACTAAATATCCTCAGTAACTGCGACCTGTCTCTTATACACT11.1235955056179776No Hit
CTGTAATCCCGCCCCAGCAAATTCTGCTGCCGCTCGGCAAAACCATCAACC11.1235955056179776No Hit
TCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCTTCTGCTTGA11.1235955056179776Illumina Paired End PCR Primer 2 (96% over 29bp)
CACCTGAAAGCTCTAGAACAAAAAGAAGCAAATACACCCAAGAGACTGTCT11.1235955056179776No Hit
ACTGAACCCCGAGGCAATGAGGAGGCTCAGATTTTCCGGCCCTTGAAATTC11.1235955056179776No Hit
GACTGTGCTGTCCTGATTGTTGCTGCTGGTGTTGGTGAATTTGAAGCTGGT11.1235955056179776No Hit
ATCTTTAGGTGCCTCATCCGACCAGTCCCGGTAGTGTTTCGTCTCTTAGCC11.1235955056179776No Hit
GACTAGGCGCATCTTCTATTGCCAGTAGATGAGGCAGTCCAGTTAGCAGAA11.1235955056179776No Hit
GAACCCGGCTGTCTTCCCTCACCTGACTGTGGTACTTCTGGCCATCGGCAT11.1235955056179776No Hit
GTTACAGGAAGTCCCTCACCCTCCCAAAAGCCACCCCCACTCCTAAGAGGA11.1235955056179776No Hit
ACTCTCACCTCTCAGACCATCTGTAAACTGACTTTCAGCCCAAAAATGCTG11.1235955056179776No Hit
ATGTCAGTTCCACCATTGTTAAATAAGTTTGCCCCATGTCAAAAAAAAATG11.1235955056179776No Hit
TGGTTACTATGGAGTCACACAGTCCACAGTACTTAGACCTGTCTCTTATAC11.1235955056179776No Hit
CCTATACACTTGGTATTGTTTGGCCCTTAAGCTTTTACATCTCTTAGCATG11.1235955056179776No Hit
AATCAGTGCTGGCTACGCTCCTGTTCTGGATTGTCACACAGCCCACATAGC11.1235955056179776No Hit
GTCGTTCACATCCACATCTCCCAGACCACACATGAGAAGCTCTAGCTCATT11.1235955056179776No Hit
GGCCAGCACTGCAAAGAAAAAGAGAAAAAAGAGAAATCAAGAGGTTCCGGC11.1235955056179776No Hit
GGTGACAGGAGCCAGACATCGGGGACTGGGGCCTGCCTGCCACTCTGATAC11.1235955056179776No Hit
GCTGTGACTACACAGTACACATGCAAAGAAGCAGTTCTGGACAAGCTGCTC11.1235955056179776No Hit
TGATTAAAGTTAGCATGGAGAGGGGAGGCAGGGCCACAGAACAGCTCTTTT11.1235955056179776No Hit
GGGACATTCTGATATTTAATGAGCAATGCTTTGAGTTTGTAGAAGCTTTCA11.1235955056179776No Hit
ATCTATTACAAACTTTAAGACTTGAGAGTGAATTTGTACTGGAGATTACCA11.1235955056179776No Hit
AACAAGCAGAATACCATTTGAAGAGTCTCAATAATTGATCTTTCTCTCTCC11.1235955056179776No Hit
CTTTTCGGTTCTTAGAGGACACATTGTGAGCAATCTCAGCACAGTAAGATT11.1235955056179776No Hit
GGAGTGGATGCACTTCTCTGTGTTGGCAGCTACAAGGACAGTACCAAGCTC11.1235955056179776No Hit
TTACCACATTGCTTCCATTCATAGAGTTTCTTTCTAGTTTGTGCTCTTTTA11.1235955056179776No Hit
CCACAAAGGCGGCTGGGGCTAGCCACATACCACCAAAGTATGAGAGTGCTG11.1235955056179776No Hit
CTCATCAATGCTGCTAGTTCAGATGTTGACAGTGTTTTGTGAATGTTGGAG11.1235955056179776No Hit
GGGATTCTCACAGTATGCACCATGTCCTTCTCTCTCCCAATGGTGGATGGG11.1235955056179776No Hit
GTTTAAGGGTAATACATTAACACGCCTCTGAAACAAGCATGGGTTGGCGCG11.1235955056179776No Hit
ACACTGTAGAACAGATTAGATGAGTGAGTTACACTGAAAAACACATTCGTT11.1235955056179776No Hit
CATTACATCTTGTCTTGGATTGAAGATCAAAATAGAAACAGAATAACTTTC11.1235955056179776No Hit
TCTTTAAAATGTCCGACGTCATGGTTTTTAGGGGTATCAAGCGGGGGTCGT11.1235955056179776No Hit
ATTTTACTGGTGTTTTCTTTATATCAGCTTCTCAGCTCAACTCTCTATAGG11.1235955056179776No Hit
GCCAGCGGTTTCGACTGGGCGGGAGCCATGCGCTTCGGCAGCACCATCTCT11.1235955056179776No Hit
TTCCTGTACCAATGAGTATTATGGCTCCTGCTCCAACTGTCCTAGTACCTA11.1235955056179776No Hit
GGACATAATGTTTATATAATGCCCTTCTTATGTGTTACCATAGATGTGTGA11.1235955056179776No Hit
TCCTGAAGGCCTCCCATGCCAGTCAAATATATACCTGTAAGGAGAGGGAAG11.1235955056179776No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCTTCTCCGGCAAAGAA11.1235955056179776No Hit
TCATTACACTTTGTTTCCCCAGCCACCTAGAACTTGAGATGTGTCCATCTC11.1235955056179776No Hit
CCTCCCCGAAGCCGCGGCCGCCCGAGGGAGAGCTATTCGAGCCGCGACTAC11.1235955056179776No Hit
CTCCTTCCTTATCCTTCCACCAGAAGAGAAATGTACCACAGTCCTGATGTG11.1235955056179776No Hit
GGGGGTGGAGCTCCATCATCAAACGGCCAATCTAGAACGTGGATTCCTTCT11.1235955056179776No Hit
CAGTCTAACCCAGCCCTTTGAAATTATCCATAGTTTTACCTGTCTCTTATA11.1235955056179776No Hit
TAGTTGAATAGTGATGTGTTTGTGCATCAAATTGAGAAGGGGTTGTTAGTA11.1235955056179776No Hit
GCGCTGCCTTGTGCCTTGGCTATGTGGGAGCTTGGTCACCTCGACCCCAAG11.1235955056179776No Hit
CAAAAAACCAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAA11.1235955056179776No Hit
CCCCTGTATTCTAGGACTCAATTAGCTCCCAAGTTGGGAGAAGCAGGAAGA11.1235955056179776No Hit
GCTCTGCCAACCGAAGCCTAAGGTCCAAACCAAGGCAGGGGCCAAAGCTCC11.1235955056179776No Hit
CTGCAGAGTGAGACTCCTAAGAAGCCTCATTACATGAAAGTGCTAGAAATG11.1235955056179776No Hit
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAACACACACACACACACACAC11.1235955056179776No Hit
CTTATACATATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC11.1235955056179776No Hit
ATAATTAACTTTGCCTATCACTTCCCGAGGCCAGAGGTCAGTGTCTCCTTG11.1235955056179776No Hit
TTATGTTCACTGTTGCTATGCTTAGACTTTTCCCGGTCCCTATCCTTATCC11.1235955056179776No Hit
CACAGTACCTAGTGTCTGTAGTGGCGCTCCCCGCTCAGTCTCTCCCATCAC11.1235955056179776No Hit
GTCAATGTGTTTCCTACGGAAGTCTTTGGGGGTTGATGTACTGCAAGATAC11.1235955056179776No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC11.1235955056179776No Hit
GCCCTGCATAATTCAGGAAATGAGTTTACTCTATCTGTGACACACATCGTG11.1235955056179776No Hit
GGGTAGGTGCAATAATAAATAAGGATATAGAGGTTGTTAAAGGGCGTATTG11.1235955056179776No Hit
CCAGGCACTCCAGCACTCTCCGCAGGGCTTCACCAGCACCCATTGGCCTAT11.1235955056179776No Hit
GGAGAAGGAGGAGGAGGAAGAGGAGAAGGAGGAATAGAAGGAGGAGGAGAA11.1235955056179776No Hit
CCTAATGTCCTTATGTCTGGAACAAGCGGGCGAGGGTGAACTCACTTAGAC11.1235955056179776No Hit
TCTGAAGAAAGGAGAATGGCGTTCAGCAAAGGATTTCGAATCTACCACAAA11.1235955056179776No Hit
GTGTAAGCTTCACACCAGGCCTCAGAGTCTGAGTATAGCCCACTCCAATTA11.1235955056179776No Hit
AGTAGATTCTGTGAGAAGAGAAGTGAAGACTGAAATTTCTTTGGTGGCAGA11.1235955056179776No Hit
CTACCAACCACCTCACTGACTGCTGACCCTGACAGTTCACACTGCCAATGA11.1235955056179776No Hit
GTAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGAGGGTGTCAGATCT11.1235955056179776No Hit
ATTTATGATGGAGCGAAAGCCCATATCAGAGTCACATGGGAAACAGAAAGA11.1235955056179776No Hit
GCGTGGCTCGCTCTCCCCGTACTCTGCGTTGATACCTGTCTCTTATACACA11.1235955056179776No Hit
ATGCAAAGATCTAGAAGGCACAGGACCCCTAGTCTCATAGCAGCGCAGTGA11.1235955056179776No Hit
TGGGTACTGAGGTTCATCTCCCAGGGCAGGGCTGGCTTTCTGTAAGTGCTG11.1235955056179776No Hit
CCTTCAGAGCGTTTTCTGTCCTACTTCAGACTTTACTTGTGGTGTGACCAT11.1235955056179776No Hit
CAATGCTAGTGGCCGTGCCTACCCAGATGTTGCCGCACTATCTGATGGCTA11.1235955056179776No Hit
GCTACACTGAGCTTAGCAGAGTGGAGCGTGGGAGGGCGTCTTAGAGCCTGG11.1235955056179776No Hit
CTCTTAAAGGGGAAAGGAAAGGTAACCAAGGCAGGAAAACATCTGTCTCTT11.1235955056179776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATT50.045.02
TGAGAAG50.045.012
TGTGAGA50.045.010
GAGAAGT50.045.018
GAGAAGA50.045.013
TGGCAGA50.045.045
GAAATTT50.045.032
AAATTTC50.045.033
AGAAGTG50.045.019
TTTCTTT50.045.036
AGAAGAG50.045.014
ACTGAAA50.045.029
AATTTCT50.045.034
AGTAGAT50.045.01
GAAGTGA50.045.020
TTCTGTG50.045.07
AAGAGAA50.045.016
GAAGAGA50.045.015
GAAGACT50.045.025
GTGAGAA50.045.011