FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_95.3410000000748d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_95.3410000000748d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437216
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC11220.2566237283173534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1250.030.59992238
GTATCAA2000.030.3783971
TATGCCG1500.028.49992843
CTCGTAT1350.028.33326139
TCGTATG1350.028.33326140
ACGAGAC1500.025.49993522
ATCTCCG1600.025.31243510
AATCTCG1450.024.8275236
CGTATGC1550.024.67735741
CGAGCCC1550.024.67735715
CCACGAG1600.023.90618920
TGCCGTC1600.023.90618945
GCCCACG1700.023.8234718
ATCTCGT1800.022.49994337
TCTCCGA1800.022.49994311
TCAACGC2600.022.499944
ATGCCGT1850.021.89183644
CCCACGA1750.021.85708819
GATAATC1650.021.81812933
AACGCAG2800.021.6963736
Produced by FastQC (version 0.11.2)