FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_94.34100000007470.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_94.34100000007470.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648092
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC15500.23916357554174408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG2100.037.5022536
ACGAGAC2250.034.999422
TATGCCG2300.034.23854443
CCCACGA2300.034.23854419
CGAGACC2350.033.51006323
CGAGCCC2450.033.06065815
CGTATGC2500.032.40194741
TCTCGTA2600.030.2902838
GCCCACG2600.030.28794118
ATGCCGT2600.030.28794144
ATCTCCG2650.029.71647310
CACGAGA2650.029.71647321
TCGTATG2850.029.2122840
CCGAGCC2700.029.16616614
CCACGAG2750.028.63587220
ATCTCGT2800.028.12668837
CTCGTAT2800.028.12668839
TCCGAGC2850.027.63110513
TGCCGTC2950.027.45715545
TAATCTC2900.027.15680135
Produced by FastQC (version 0.11.2)