FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_93.34100000007463.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_93.34100000007463.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472266
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC35560.752965489787535No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT5230.11074267467910033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4900.035.35473343
TGCCGTC4900.035.35473345
TCTCGTA4850.035.25532538
AATCTCG4850.034.79144336
CTCGTAT4950.034.543139
CGAGACC4950.034.543123
CGTATGC5000.034.19766641
ATCTCCG5150.033.65273310
TCTCCGA5150.033.65273311
ATCTCGT5150.033.6384837
ATGCCGT5150.033.6384844
CACGAGA5100.033.52712221
CGAGCCC5200.033.3220915
GCCCACG5200.033.31503318
ACGAGAC5150.033.2016222
CCACGAG5200.032.8823720
GATAATC5150.032.76475533
CCCACGA5250.032.56920619
TCGTATG5250.032.56920640
TCCGAGC5350.032.38782513
Produced by FastQC (version 0.11.2)