FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_90.3410000000743d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_90.3410000000743d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2257
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC124255.02879929109438No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT1446.380150642445724No Hit
TATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTT150.664599025254763Illumina PCR Primer Index 7 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT110.48737261852015945No Hit
ATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTTC100.4430660168365086Illumina PCR Primer Index 7 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC70.31014621178555607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.2215330084182543No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC50.2215330084182543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.2215330084182543No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC40.17722640673460346No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC40.17722640673460346No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC40.17722640673460346No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.1329198050509526No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG30.1329198050509526No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC30.1329198050509526RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACCCTGGATAATCTCGTATGCCGTC30.1329198050509526No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCAGTC30.1329198050509526No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC30.1329198050509526No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT205.6362164E-445.045
CTTATAC1700.041.029411
TGCCGTC1750.039.85714345
ATGCCGT1750.039.85714344
GTATGCC1800.038.7542
TATGCCG1800.038.7543
CGTATGC1800.038.7541
ATCTCGT1750.038.5714337
TCTCGTA1750.038.5714338
TCGTATG1750.038.5714340
GACCCTG1750.038.5714326
AGACCCT1750.038.5714325
CTCGTAT1750.038.5714339
GAGACCC1750.038.5714324
AATCTCG1700.038.3823536
GATAATC1700.038.3823533
ATAATCT1700.038.3823534
GGATAAT1700.038.3823532
CTGGATA1700.038.3823530
TAATCTC1700.038.3823535
Produced by FastQC (version 0.11.2)