FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_9.34100000006f28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_9.34100000006f28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511453
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC11420.22328542407611257TruSeq Adapter, Index 12 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1350.029.99980543
TCGTATG1500.029.99980540
ACTTCGC300.005144783329.99980428
CGAGCCC1450.027.93085315
CGAGACT1450.027.93085323
CGTATGC1500.026.99982541
CACGAGA1500.026.99982521
GTATGCC1600.026.71857542
TGCCGTC1550.026.13141445
CCGAGCC1550.026.1288614
ATCTCGT1600.025.31233437
GCCCACG1600.025.31233418
CCCACGA1600.025.31233419
AATCTCG1600.025.31233436
ATGCCGT1600.025.31233444
ACGAGAC1600.025.31233422
TCTCGTA1650.024.54529438
GTATCAA3450.024.1349951
ATCTCCG1700.023.82337610
TCTCCGA1700.023.82337611
Produced by FastQC (version 0.11.2)