FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_87.34100000007406.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_87.34100000007406.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551519
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC18310.3319921888457152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGTT250.00210651435.99876830
CTCGTAT3100.035.56652539
TATGCCG3300.033.41097643
TCTCGTA3350.032.91230838
TGCCGTC3350.032.91230845
GCCCACG3450.032.6075818
CGTATGC3400.032.428341
TATCTCG3400.032.428336
TCGTATG3400.032.428340
CCCACGA3400.032.4253619
ACGAGAC3400.032.4253622
CGAGCCC3600.031.2489315
ATGCCGT3550.031.0580944
CGAGACA3450.030.65112323
ATCTCGT3600.030.6267337
TAATATC3550.030.42425233
CCACGAG3700.030.40436620
TCTCCGA3600.029.99897411
ATATCTC3550.029.79041335
CCGAGCC3800.029.6042514
Produced by FastQC (version 0.11.2)