Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 12h_86.341000000073f8.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 55141 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 37 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC | 2355 | 4.270869226165648 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 797 | 1.445385466349903 | No Hit |
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT | 452 | 0.8197167262109863 | RNA PCR Primer, Index 44 (95% over 22bp) |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 172 | 0.31192760377940193 | No Hit |
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA | 123 | 0.22306450735387462 | No Hit |
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC | 111 | 0.201302116392521 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 111 | 0.201302116392521 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT | 107 | 0.19404798607206977 | No Hit |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 72 | 0.1305743457681217 | No Hit |
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 67 | 0.12150668286755771 | No Hit |
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG | 62 | 0.11243901996699371 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTATCA | 45 | 1.8741048E-8 | 39.994354 | 1 |
CCAGCTC | 25 | 0.0020912308 | 35.99492 | 40 |
GCCGTCT | 80 | 1.8189894E-12 | 33.745235 | 45 |
TGCCGTC | 300 | 0.0 | 32.995342 | 45 |
TAAGTAA | 30 | 0.0051088575 | 29.995764 | 16 |
ATTGGTT | 30 | 0.0051088575 | 29.995764 | 3 |
AACTTAT | 30 | 0.0051088575 | 29.995764 | 2 |
TGTACCC | 30 | 0.0051088575 | 29.995764 | 27 |
GTAGTTA | 30 | 0.0051088575 | 29.995764 | 13 |
ATGCCGT | 340 | 0.0 | 29.113537 | 44 |
ATCTCGT | 340 | 0.0 | 28.451866 | 37 |
TCTCGTA | 340 | 0.0 | 28.451866 | 38 |
TCGTATG | 340 | 0.0 | 28.451866 | 40 |
ACGAGAC | 350 | 0.0 | 28.281721 | 22 |
TATCTCG | 335 | 0.0 | 28.204975 | 36 |
CTCGTAT | 345 | 0.0 | 28.03952 | 39 |
CCACGAG | 355 | 0.0 | 27.883387 | 20 |
TATGCCG | 355 | 0.0 | 27.883387 | 43 |
CACGAGA | 355 | 0.0 | 27.883387 | 21 |
CCCACGA | 355 | 0.0 | 27.883387 | 19 |