FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_86.341000000073f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_86.341000000073f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55141
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC23554.270869226165648No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7971.445385466349903No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT4520.8197167262109863RNA PCR Primer, Index 44 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.31192760377940193No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGA1230.22306450735387462No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC1110.201302116392521No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.201302116392521No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT1070.19404798607206977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.1305743457681217No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.12150668286755771No Hit
AAAAAGTACTCTGCGTTGATACTGTCTCTTATACACATCTCCGAGCCCACG620.11243901996699371No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA451.8741048E-839.9943541
CCAGCTC250.002091230835.9949240
GCCGTCT801.8189894E-1233.74523545
TGCCGTC3000.032.99534245
TAAGTAA300.005108857529.99576416
ATTGGTT300.005108857529.9957643
AACTTAT300.005108857529.9957642
TGTACCC300.005108857529.99576427
GTAGTTA300.005108857529.99576413
ATGCCGT3400.029.11353744
ATCTCGT3400.028.45186637
TCTCGTA3400.028.45186638
TCGTATG3400.028.45186640
ACGAGAC3500.028.28172122
TATCTCG3350.028.20497536
CTCGTAT3450.028.0395239
CCACGAG3550.027.88338720
TATGCCG3550.027.88338743
CACGAGA3550.027.88338721
CCCACGA3550.027.88338719