FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_85.341000000073eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_85.341000000073eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237491
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC25491.073303830460944No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT3010.12674164494654536RNA PCR Primer, Index 44 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT3350.034.92521344
TATGCCG3450.034.56505643
TCGTATG3650.033.9039540
TCTCGTA3600.033.7498438
GCCCACG3550.033.59139318
TGCCGTC3500.033.42841345
CTCGTAT3650.033.28751439
TATCTCG3450.033.26071536
ATCTCGT3600.033.12484437
CGTATGC3600.033.12484441
ACGAGAC3650.032.6710822
CCACGAG3650.032.6710820
CGAGACA3450.032.60854323
CTCCGAG3750.032.3998512
CGAGCCC3750.032.3998515
CACGAGA3750.031.7998521
GCCGTCT855.456968E-1231.76455745
CCCACGA3800.031.3814319
TCCGAGC3850.030.97388313
CCGAGCC3850.030.97388314
Produced by FastQC (version 0.11.2)