FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_84.341000000073de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_84.341000000073de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239288
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC27591.1530039116044266No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT3470.14501354016916854RNA PCR Primer, Index 44 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGA250.002103926435.9994962
TGCCGTC4150.035.24047545
TATGCCG4200.034.82094243
CGTATGC4250.034.41128541
CTCGTAT4300.034.01115439
TCTCGTA4300.034.01115438
TCGTATG4300.034.01115440
GCCCACG4350.033.62022418
CCACGAG4350.033.62022420
TATCTCG4300.033.48790736
ATGCCGT4400.033.2381744
ACGAGAC4400.033.2381722
CGAGACA4300.032.96465723
ATCTCCG4450.032.8647110
CTCCGAG4450.032.8647112
TCCGAGC4450.032.8647113
TCTCCGA4450.032.8647111
ATCTCGT4500.032.49954637
CACGAGA4500.032.49954621
CGAGCCC4500.032.49954615
Produced by FastQC (version 0.11.2)