FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_83.341000000073c1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_83.341000000073c1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281215
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC31281.1123161993492523No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT4150.14757392031008304RNA PCR Primer, Index 44 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC4450.036.4014722
TGCCGTC4450.036.4014745
TAATACG250.002105265635.9970134
ATTAGGC250.002105265635.9970133
CGAGCCC4500.035.99701315
TATCTCG4450.035.89589736
CACGAGA4550.035.6014421
TATGCCG4500.035.49705543
ATCTCGT4600.035.2144737
CTCGTAT4550.035.10697639
TCTCGTA4550.035.10697638
CCACGAG4650.034.83581520
ATGCCGT4600.034.7253844
TCTCCGA4700.034.47135511
CGAGACA4550.034.11804623
ATATCTC4700.033.9865435
GTATGCC4700.033.9865442
TCGTATG4700.033.9865440
CCGAGCC4800.033.747214
CGTATGC4750.033.62878841
Produced by FastQC (version 0.11.2)