FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_82.341000000073b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_82.341000000073b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382966
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC17740.46322650052485076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC1850.032.83745622
TATCTCG1800.031.2496436
CTCGTAT1900.030.78911839
TCGTATG1850.030.40505240
ATCTCCG2050.029.63380210
TATGCCG1900.029.6049243
CCACGAG2150.029.30198720
ATAATAT1850.029.1888532
TCTCGTA2050.028.53625538
CACGAGA2150.028.25548721
CCCACGA2150.028.25548719
TGCCGTC1950.027.69198645
GTATGCC2050.027.43870742
TCTCCGA2250.026.99968511
CGTATGC2100.026.78540241
ATCTCGT2200.026.59060137
ATGCCGT2150.026.16248944
CGAGCCC2350.025.85076315
CGAGACA2200.025.56788623
GCCCACG2400.025.31220818
Produced by FastQC (version 0.11.2)