FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_81.341000000073a8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_81.341000000073a8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1487
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC73949.697377269670476No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT936.254203093476798RNA PCR Primer, Index 44 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCTTC231.546738399462004RNA PCR Primer, Index 44 (100% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT171.1432414256893073No Hit
TATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCTT130.8742434431741762RNA PCR Primer, Index 44 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC120.8069939475453934No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC80.5379959650302623RNA PCR Primer, Index 27 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCG70.4707464694014795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.4034969737726967No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.3362474781439139No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC40.26899798251513113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.20174848688634836No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC30.20174848688634836No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC30.20174848688634836No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.20174848688634836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAA30.20174848688634836No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC20.13449899125756556TruSeq Adapter, Index 12 (95% over 21bp)
AGATACTTTAGCTTAACTTATCTTAACCTTTTTCTAGCTTAATGCAAATCG20.13449899125756556No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC20.13449899125756556No Hit
CTTATACACATCTCCGAGCCCACGAGACAACAAAATATCTCGTATGCCGTC20.13449899125756556No Hit
TCTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC20.13449899125756556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC900.042.51
TATACAC1050.036.428573
TGCCGTC1050.036.4285745
TTATACA1050.036.428572
AGCCCAC1100.034.77272817
GTATGCC1100.034.77272842
ATCTCGT1100.034.77272837
ATCTCCG1100.034.77272810
CACATCT1100.034.7727287
GCCCACG1100.034.77272818
CCACGAG1100.034.77272820
TATGCCG1100.034.77272843
CATCTCC1100.034.7727289
GAGCCCA1100.034.77272816
CACGAGA1100.034.77272821
CCCACGA1100.034.77272819
ACATCTC1100.034.7727288
TACACAT1100.034.7727285
ACACATC1100.034.7727286
TCTCGTA1100.034.77272838
Produced by FastQC (version 0.11.2)