FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_80.3410000000739b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_80.3410000000739b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC85.228758169934641No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC63.9215686274509802No Hit
CCTCTGAACCTGTAAGCCAGCCCCAATTAAATGTTGGCCTTATTAGAGTTG10.6535947712418301No Hit
GAGCAGAGAGAGACCAAGGGATCATCCTGAAACAGGTCTCTCTGCACTTCC10.6535947712418301No Hit
ACTTTGATTAGTACTGAGTCTTTTCATTAACTGCTTACTACCACAAGAAAA10.6535947712418301No Hit
TATCCATCCTGTCTATGCTACCCATTTGTTAGCTGTCTCAGTTATCAAATC10.6535947712418301No Hit
ATGAAGACTTGCACAGTGGACTCGGCAGCACCCTTGGTGAACTGGCGCATC10.6535947712418301No Hit
CCCTGACATAATGCCATGTCCTTAATACCAAAAAAGGAGCTCTTAGGCATG10.6535947712418301No Hit
GAACAGAATTCAAAAGTCCTGACTCAGAAAGTTGGCCCATCCAGAAATATA10.6535947712418301No Hit
GGGAAGGAGGGCCAGGCCTGCTGAACATATAAGACCTCTGCAGTGACCACA10.6535947712418301No Hit
CCATTGGTGGGTCGTTTTTGCTGTCACCAGCAACATTGCCTCGTCTAACAT10.6535947712418301No Hit
GTTACCGCTGGTAAAGCTGTCGGCTGGTTTGCCTGATTTGAGCGCTTGACC10.6535947712418301No Hit
GTGTTCCTCCCTGCCCCAAGCATCCCTGTCAGTCCCAGTTGAAGCTGTCTT10.6535947712418301No Hit
GTGTGGTGTGGTGCTGAAAAAAAAAAAAAAAAAAAAAAAAAAGTCCTCTGC10.6535947712418301No Hit
CCGTCGGCAAGACTTACCACCAACACAATCACACCGTCTCTCGCGGTTTCA10.6535947712418301No Hit
ATGGTGGGCTCAGTCGTTCCTCCCGGGGGTCCCACATGTTGTTGCCGGCTT10.6535947712418301No Hit
GCTTCAACCAGATTCCTGAAAAGGCCTCTTGATATATCAAGATAGAACCTG10.6535947712418301No Hit
ACCAGAAGGCAATTGGAAATGCCCTGAAGTCCTGGAATGAGACCTGTCTCT10.6535947712418301No Hit
CCCTGAGCACTGGAATCAAAAGCATGAACAGCTTTTAGAAGACGGAAGAGG10.6535947712418301No Hit
ATGGAGTGTCACGTGATAGTTCAAGGAAGTGAGGACGTATCTGTTCCTAGG10.6535947712418301No Hit
GCTCCACTTCGGCCATCTTGGCGGTTACTCGGAAAAAAAAAAGCACCCGTC10.6535947712418301No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTG10.6535947712418301No Hit
GTGCCAGTGGCACCACGCTGCTGGAAGCTTTGGATTGTATCCTACCACCAA10.6535947712418301No Hit
CTTGGTTGTACTTTGGTTTACTCTTAAGTAGTAATGACTCTGCTAACAAGA10.6535947712418301No Hit
GGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAAGTCGAACGGAA10.6535947712418301No Hit
CTTTATGATCTGATGTGAAATGACCAAGTCAAAGTCTCCTTTTAGTTCTTT10.6535947712418301No Hit
GCACAGCCCTGAAGGCCAATCCGTTTGGGGGTGCCTCTCATGCAAAGGGAA10.6535947712418301No Hit
GCGCTGCAGGTATCACAGTGCCAGCTGCTAGTTTCCACTGAGCTTCCTCAT10.6535947712418301No Hit
GTTGTGTCCACCACCCAGCCGGGTCTGTGAGTCTCACCTGTCACGTGACAT10.6535947712418301No Hit
GTACAAAGGAGGAAAAGCTGACCAGCAAGAAGAACGTCGGCGACAGAAGCA10.6535947712418301No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCC10.6535947712418301No Hit
CCTATTATCACTACCAGTGCTAGCCGCAGGCATTACTATACTACTAACAGA10.6535947712418301No Hit
AGCATTGACAGTTTACACTGGGCAGCTGGAGGCCTGATAAGACGGTGTCTG10.6535947712418301No Hit
CGCCCCCAGCGAGTTCGAGAAGGCGACGACCGGAGCGTCCTCGGGACCCTC10.6535947712418301No Hit
GTGACAGACAGAGCCTGCTCCTCAAAGTTGGCCTGATTGCTACAAACTCTA10.6535947712418301No Hit
GGTATATGTTCTGCCACTCATTCACGAGATCGTCGCCCTTGGTAAGCTTGT10.6535947712418301No Hit
CTTATAACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT10.6535947712418301No Hit
AGATTGAGGAGCGGCACAAGCGACACAAGGAGCGCATGAAGCAGATGGAGA10.6535947712418301No Hit
GTTAAAGCCAAGGAAGGTGTGGTAGAAATGGTCAGATGTTTATTCTGTCTG10.6535947712418301No Hit
TCACTGAGCTAGTCAGATGAGAGAGTAGCACCCTGTCCCTCAGAACCTGTC10.6535947712418301No Hit
TGACTGCTGCAAGAAGGACAAACAAGTGTCAGCGTCACAGCAGGCACCAGG10.6535947712418301No Hit
GTTACAGTCTATATTCAAACGAGGGTTGACAGTATGTTACATTCACTTACA10.6535947712418301No Hit
ACTCAGCGGGTCTGTGAACTCCTCCCAACTGGGCTCAAAGACCGGGCACTC10.6535947712418301No Hit
GTGGAGGATTGTCGTCTTGAATAGAGTCTAAAGTGCTGTCTCTTATACACA10.6535947712418301No Hit
CCTCTGTATAGTATCCCCGTGGCTCCCCCAGCAGCCCTGACCCACCTGGCT10.6535947712418301No Hit
CCGGAGGACCCTGCAGATTCTCCGGGAAAACCTGGAGGAGGAAGCCATCAT10.6535947712418301No Hit
GGTCAACAGTAAAAAACACTTGTTGCTCTTGCAGAGGACCTACGTTCAGTT10.6535947712418301No Hit
AAGCTGCACCTCATTTTTCTCACAAGTTCTTACAGGTCACTCTGGAAGCTG10.6535947712418301No Hit
AAACAAAATCATAGCCAAGGGGAATTATTGTTCTATAGTTGCTGTCTCTTA10.6535947712418301No Hit
TCTCTGAAGTATTGAAGTTAGAGGGAACTCAATCAGGATACTGGTTATGAA10.6535947712418301No Hit
GCGTTAGTCTGAGACCTCATGTTACTTAGGCCTTCAGCAAGGCCCGTCACA10.6535947712418301No Hit
GGGCCAGATCGGCGGCCGGGCCGTCGTAGATCGGCGCGGGCAGTTTGGTGA10.6535947712418301No Hit
TCGCCAGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCT10.6535947712418301No Hit
CTTAAATATTTTCACACCAAAAACCTTTGTTAAAAAGTATGGTATCAGCCA10.6535947712418301No Hit
GTATTTGCTGGTCTCGAATTTCCACAGGGAGATGTCAATAGTGATACCACG10.6535947712418301No Hit
GGCCTACCCATTCCCCTAGTTTCTTGTTGTCATCAACCTATCAAAGACAAA10.6535947712418301No Hit
GTACTTGGCCTCCTCGGGCGTGATGCGGTGTACCGCGAAGCGGCCCTTGGT10.6535947712418301No Hit
CTTCTGGATGTAATGTGTACCACACTCATGAGATCTTAGTGGCTATGGTTA10.6535947712418301No Hit
TTTTTAGGCTGGCTCTCTGCAAACTTCTTAGCATCAAACTGAGTCATCTTC10.6535947712418301No Hit
ACCTACACTCCCGCCTGCTGGAGAGAGCAGCCAAGATGCACGATTCCTTTG10.6535947712418301No Hit
GTAACCTACTGTTCATGTTTGCTCTGGTCTGAAGTGTTTAGCTGTTGAGCT10.6535947712418301No Hit
ATGATAATTACTCCATTGCTTGCTTGTATTTTTTTCTGTAGTGCTGGGGAA10.6535947712418301No Hit
CTCTCACACTGTGCATCACAAAGCTAACAAATGGAAATCTGAATTGCAGTC10.6535947712418301No Hit
GAAGTACATTGGTAAGATTGAGCAGTATTAAGATGAGGTACATAGCAAGTT10.6535947712418301No Hit
GCATCCATCTTACCAGAATTGAAGAAGGAAGCATACGGCCAGATCCGAGTG10.6535947712418301No Hit
ACGTCATGGGCAATCACCACCAGCTGAGCCTTCTTGTTCTCCACCAAGGTG10.6535947712418301No Hit
CTACCAAGAGGCGTTTGATATAAGCAAGAAGGAGATGCAGCCTACGCATCC10.6535947712418301No Hit
GGTAGAAGAGAGTGCCTCACAGAAGACAAGTGCAGAAATGGAAGCATATCC10.6535947712418301No Hit
CCCCACACACGGTGCGCAGCTCGGGTTGACGAGCCCAACCGAAGAAATCGG10.6535947712418301No Hit
GTATAGCCCTGGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCCTCGA10.6535947712418301No Hit
ATACCTCCCCTAGGACTCCGGGGAGAAGGCAAGTGCTGGAGTCAATTTACT10.6535947712418301No Hit
ACACCATTTCAACAGTGATTTCAGTCGACATACTTTCCAAGAATGTCACTA10.6535947712418301No Hit
GTTTCAGTAAGAACAATACAGATCTGTACCTGCGGCTCCAGTCAGATATCC10.6535947712418301No Hit
GCGTAGATGGGCTGCCGGTGTCCACGAGCATGGGCACCTCGCTCTTTTCCG10.6535947712418301No Hit
CTCTTGTTGCCTGAGATGACCCTGACTCCTGCTTCCCCTGCCTTTGCCTAT10.6535947712418301No Hit
TCCCTCAATTCTCTGCGCCCCTCACCGCAACCACCAGCACGTGGCGGCAAC10.6535947712418301No Hit
TCCCATTCTTGAACTGAGGGGAGAAGGGCCCGTGCTGGTCTCCTTTTCTCT10.6535947712418301No Hit
GGGCTAAGACTACGGAGGTGGGGGATTCCCAACTTACTCTCCACCCTTCTT10.6535947712418301No Hit
ACTGGAAACCCTGACTTTATCACAGAAGGGCGGCAGCATCTCTGGACTTTG10.6535947712418301No Hit
GGCTAAGGCAGGACAATTGCTTGAACCTAGGGAGGGTGGAGGTTGCAGTGA10.6535947712418301No Hit
ATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCTTCCTAACATTA10.6535947712418301No Hit
CAATACCATCACCAATGCTTAACACACGCCCAGTCTCCTCCAGGTCAACAG10.6535947712418301No Hit
GCGTGTTCCCATTACCGCTGGATCAGGACGGACAGGGCTGCTGAGAGGTCC10.6535947712418301No Hit
CCAGAAGGCTGTCGAACCCTTGTATACAGAGGTATACATCAAGAGCTGGTG10.6535947712418301No Hit
CAGAGAAGAGACTGAGGTGGCAGCTGCCCAGAATCTTTGTGGGTTACAGAT10.6535947712418301No Hit
CCACTACTGCAAGTGTGCCCTGCGGAGAAACCAGGCAGGCGCTACTGCCTC10.6535947712418301No Hit
CTCTGCATCAGTTCCTGCTTTCTGACCTGTTTGAGTTCCAGTCCTGACTTC10.6535947712418301No Hit
GTGCCACTATACCCTGCAAGGTTTCCCTTCTTAATCAGCATAGCTGTCTAG10.6535947712418301No Hit
GCCAGTGTTACTGTTGTGACTGTTGGCTGAGAGGAAGTTTAGATGAGGTTC10.6535947712418301No Hit
TGCAAATGGTTGTAAAATGCCGTATGGACCAACAATGTTAGGGCCTTTTCG10.6535947712418301No Hit
CATCATAACAAAACAACAACAACAACAAAACATGAAGAAAGTCTGTCTCTT10.6535947712418301No Hit
CCTGTGCAGGCACATGTTCTTAAGGAATGCCATGCACACAGGCCTGAAGAG10.6535947712418301No Hit
CCTCAGCAGAGTCATCTGCACCTGCCTCAGACTCCATCTTCACATTAGTCT10.6535947712418301No Hit
ATCGTGGTTTTACCTGCATTCTCCTGTGCATTCTGCACCCTGAGGCTCTGA10.6535947712418301No Hit
CATAGAAGAGATAGTGTTTTTCTACTTTGCCATCCTCAGTTTTCATTTCTG10.6535947712418301No Hit
CTCTATTTCCATGTTGGGCACAGGCTCACTTCCCTTTTACTGTCTTGTCAC10.6535947712418301No Hit
AATGGGAATCAAGACTATATTGCCAATTCTTTCTGTTTGGAGACTGTCTCT10.6535947712418301No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACAC10.6535947712418301No Hit
TATGTAGAGTGTCTCATTCAAACTTGAGAAAAGTTGTGTGCATGTGCGTGT10.6535947712418301No Hit
ATGCAAAAGTTCAGTACAAGCCATTTGTAGGGCTTGGGATTCTTTTAAAAA10.6535947712418301No Hit
CAGAAGGCCAGGCTGGAGAGCTGCTGGGCCCCACGCCATCCTTCACTGGGG10.6535947712418301No Hit
CTGCTCTGCTCGCTCAATCCTGCTAGCTCTGGCCCAAAGATTTATTTATTA10.6535947712418301No Hit
GTGCTGTAATGAATAGGTAGTTTCATTTCCTGAAGGTGTTCTGTTAGAAAA10.6535947712418301No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTGT10.6535947712418301No Hit
GTGTGAGATAGTTCAGCTGGTAATGCTCATCATGGAGCCTGGCGGCCTGAG10.6535947712418301No Hit
CATTGGGGTCCTTGAACTTCTTTTTGGTCTCCCCTTTGGGGGGCTGTCTCT10.6535947712418301No Hit
GCCCGCGACCACGGACTTCGACCTCGACACGTCAGTGGACCTGTCTCTTAT10.6535947712418301No Hit
CCAGAAGAGGGCGTCAGATTTCAATATGGCTGTGAGCCACCATGTGGTTGC10.6535947712418301No Hit
CCTTAATTGATCGTGAAAAAGAAAAGAACTTGCTTGCATCCCATTATCATC10.6535947712418301No Hit
TCTCTGAGATGGGGGTGTCGATGATCCTCTTGTCCTGTCTCTTATACACAT10.6535947712418301No Hit
ATAGCAGAATGTGTTATTTCTTTTTTACTGTTTCTGTTTTGTTTTGTTTTG10.6535947712418301No Hit
TTACAAGAAACCAGCTTCACAAAAGAGGCTTACAAAAAGTACATCAAAGAC10.6535947712418301No Hit
GATGGGACCAATAATTTTGTAGCCCAGCAGGAAGATTTTGCTGTCATGATG10.6535947712418301No Hit
CCGCTGCTGCTGTGGCTTAGGCCGCCTCCCCAGCCGGGCGGGCACCTGTCC10.6535947712418301No Hit
CGATTATCCTGCCTCAGCCTCCCAACTAGCTGGGCTGTCTCTTATACACAT10.6535947712418301No Hit
TAGTAAGGAGGGTCCCTTGGATTTTAGGCTTAAAGTACCTTAAACTACATC10.6535947712418301No Hit
ATGGTAGGGAATCTGCCACAAAATGAAACTCATCTGGTGTGATACTCTGGC10.6535947712418301No Hit
TTATTAGGGAGAGTGATTCTTTATTCAATCAATCTGATGCTTTTTTACTCT10.6535947712418301No Hit
ATTCACAGTCTATTTAACTTTAGTCTTTCTAGTTGGTGTGCAGTGGTATCT10.6535947712418301No Hit
GCGGACACTGCCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACT10.6535947712418301No Hit
GATTAAGAGGACTCTGTCTTCATGAACGGATAATTCTATCTGTGCCTTAAT10.6535947712418301No Hit
CAAAGGGGTGGCCGCCATCGCTGGCCCGAGCTGGGGCTGCGCTCGGGGCTT10.6535947712418301No Hit
CTGCTGCAGGCCTCGGGCCTCGCCCCGCGGTTCCTGGCCAAGGAGGCCATG10.6535947712418301No Hit
CATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCTTCTGCTT10.6535947712418301Illumina PCR Primer Index 4 (96% over 28bp)
AATGATTGGCATGGGAGGGCTCAGCCTGTGGGCGGTGCCACCTCTGAGCAG10.6535947712418301No Hit
CCTTAGGAATTGGAATAAGGCAAGAAAACTGAAAATCATGGAAAATGAGAA10.6535947712418301No Hit
CATCTTCTTCTTCTGGTTCATCTTCCTCTTTAACAACCTGTCTCTTATACA10.6535947712418301No Hit
GAATGGAATTGTCATCAAATGGAATTCAAAGGAAACATCATCAAATGGAAC10.6535947712418301No Hit
GCTCAGGACTTATGGGGGGGGGAGGTAAGTGATGATTTCAGCTGAGTTGGC10.6535947712418301No Hit
GATGTAGTGTACTAGGCTTCAGAGCATCGTTGCACGTGATGCCTCAGAACA10.6535947712418301No Hit
GCACTGGAGGACAGCCTGTAGGCACTGCTAGCCTCATTCCTCTGCAGTCAG10.6535947712418301No Hit
GTATAGGCTTAAGTGGCCTGTGAAACGTTCCTTCGCGGATTCTACAAAAAC10.6535947712418301No Hit
CCCACATGTTGCATGGAAATCTCCTTACATGAATTCATAAGTATGCTTGGT10.6535947712418301No Hit
AATTAAGACTGGTTGAGATTTTGGCCGGGTGTAGTGGCTCACATTTGTAAT10.6535947712418301No Hit
CTTTATGGATGGTATCACTTTCATAAACATTTCACTATGGGATTCTTAGCT10.6535947712418301No Hit
GTGTATATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCCTATTCCAGG10.6535947712418301No Hit
CAACTAACACAGTTGAACTTTTCTTTAGACAGAACAGTTTTGAAACACTCT10.6535947712418301No Hit
GCGCGGTCCTTTCCTGCGGCGCCTTCCGTCCGTCGGCTTCTCGTCTTGCTC10.6535947712418301No Hit
GTACAGCCTTGTGTATTGTCCCCATACTGATGGACGTGGAACCCATGCTGG10.6535947712418301No Hit
GCCAAGACCTCACCCAGGAGCAGAGAGATCAGCTGAGGGCAGAGATCAGCA10.6535947712418301No Hit
GATGCACAGATTTTCTCTGCATGCACAGGCAGTGCTCGGTGAGAACAAATT10.6535947712418301No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers