FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_8.34100000006f0e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_8.34100000006f0e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC43.076923076923077No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC43.076923076923077No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC21.5384615384615385No Hit
TCTATTCAAGCATACTAATCTGCTCTATGAACTCAGAAAAGGTAGAGCATA10.7692307692307693No Hit
CTCCAGCGGGATCAAAGAAAGTTGTGTTTAGGTTGAGGTCTGTTAGTAGTC10.7692307692307693No Hit
GCCCAAACCAGTTTGCTGTGCCTAGTGGACGGTGGCAGTGGCAACAGATCC10.7692307692307693No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT10.7692307692307693RNA PCR Primer, Index 27 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTGCGAAGCAGTGGTATCAACGCAGAGT10.7692307692307693No Hit
ATGTAGGCAGGACTTCTTCAGTGGTTTGTGGAGTTCAGCCTAGATCTAGAG10.7692307692307693No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTGAAAACTGTGG10.7692307692307693No Hit
CTCTGTTAGTTACACGAACTATCATCCTATATTTGGGCTGTCTCTTATACA10.7692307692307693No Hit
CTGGTGTGATGGTGTATCCAGGACTTGCTAAGGTGGGAGAGTTTGGTTCTG10.7692307692307693No Hit
AGAATGGCGAGGGTTATCTGGTTACAGTTGGAACAATCAGTATATATCAGA10.7692307692307693No Hit
CCCACAACATGGCCTTCACCCCCAGTTTGGTCCCATAGTTCCGGTCGAAAG10.7692307692307693No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATTCCGTC10.7692307692307693No Hit
GAGTAGGAATACTTCATGCTCACGACAGGCTGTGTCTGGAAGGAAAAGGCA10.7692307692307693No Hit
CTTATACACTCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT10.7692307692307693No Hit
ACACTTTCTCACTAACTGGTGGACATGACCAAGGAATCTGTCTCTTATACA10.7692307692307693No Hit
CCCCGGGAGCAGTGGTGGCTGAATCTGCGGCTGATGCTGAAGATGCTGGCA10.7692307692307693No Hit
ATCATCAACTGCAGAGGGAGGTAACTTTAAAGTCAGAACTGCGCCACCACA10.7692307692307693No Hit
GCTACACATTCACTGTCTCCCGCAAAACAAAACAAACAAAACAACACAGAA10.7692307692307693No Hit
GTTTTTACCAGTTACAGGAAGCAGTTTCTGTTCTGTCTCTTATACACATCT10.7692307692307693No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTGAAGCAGTGGTATCAACGCAGAG10.7692307692307693No Hit
TAATAATACTGCACCCTCTAAAAAGAAGGGTTGGGGCAGAGAAAATGCCAA10.7692307692307693No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCCAAAAAAAAAAAAAAAAG10.7692307692307693No Hit
CACACACACACTCACACACACACACACACACACACACACCCGCTATTTTTA10.7692307692307693No Hit
GACAAAGAGTGGAGAAGAGACTAAAGAAAAGTCCACCTAGAGACTGTGCCC10.7692307692307693No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAGTACCCTGCGTTTAT10.7692307692307693No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTGCAGTGGTATCAACGCAGAGTA10.7692307692307693No Hit
TTCTTGGTCCTTGTCACTGTCTTAAAAGGTTATATTGTTAATTTATTATGT10.7692307692307693No Hit
GGCTCAGGCAGGGAGGGGATGAAAGGAGCACGGGAGAAACCTAAGCCAATA10.7692307692307693No Hit
GTTTTACTCGCATATTTTACGTCCTAAAGTGTGTTTTTCTCATTTTCCGTG10.7692307692307693No Hit
ACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCTTCTGCT10.7692307692307693Illumina PCR Primer Index 4 (96% over 27bp)
GTCCATTAATCATCTCTGAAAAAGTGGCATTGAATTTTAGCTCTATTTTAC10.7692307692307693No Hit
CTTATACACATCTCCGAGCCCCCGAGACTAGATCCTATCTCGTATGCCGTC10.7692307692307693No Hit
CTTATAATGCTGTTTAGTTTTGTACTATTGGTGTGTTGGTGAATTTTTAAA10.7692307692307693No Hit
GTAATCCAGCACTTCCTGTGGCTGAATGGGAGGAAGCACAAAGACAGGCAA10.7692307692307693No Hit
GCACACCCTCACCTCAAGCTGTTCCTGGAAACAAAAGTAGCAGGACAATTA10.7692307692307693No Hit
CATTTTATCTATGGGCCATTGGATCCTATAAATCCGTATCCAGAGTTTTTG10.7692307692307693No Hit
TCCTCCACCAGTACCTCCAGGGACACCGATGATTCCTGTACCAATGAGTAT10.7692307692307693No Hit
CCTCAAAGCACATCTTCAAAATCCCAAACCCTTGGGAGTCAAGGTCACCGG10.7692307692307693No Hit
GGAACAAAGGCCGAGTTCCTTTAACAAGATGCTTGATCAAATTTCCTGGAA10.7692307692307693No Hit
GTCGATGTTTCTCATTTTCCATGATTTTCAGTTTTCTTGACAGATTCCAGG10.7692307692307693No Hit
ACGCAGAGTACTTTTTTTTTTTTTTCGAACCAGTGGAATAAACGCAGAGTA10.7692307692307693No Hit
CTTTACCTTTTAATCCCCGCATCTCGTTATACAATACGAATTGCCCAGTCC10.7692307692307693No Hit
ACATAAACCCAACCTTTGGCAGTTACAGACAAGAGGAATGTATATGGGTTG10.7692307692307693No Hit
CAGCCAGAGCAGGCCGTGCTCTGATGCAGAGGTGGTAACCTGTGCTCCATG10.7692307692307693No Hit
CTGAGATCGTGCCATTGCACTTCAGCCTGGGCAACAAGAGCGAAACTCTAT10.7692307692307693No Hit
TAGTAGAGCGCCTGCTCTTTTTCATCTAGTTTCTGTTCTAAGCGCAAATTG10.7692307692307693No Hit
TGTAGATGGAGCGCGGACAGCCGGCGTTTGCTGCGACTGTGCGGTCGACGA10.7692307692307693No Hit
CTTGCTGAGTCTATTCTGCACGAACTAATATTCCAATAGGAAACATTTTCA10.7692307692307693No Hit
GGGGAGCATCCATGGGCTTCAACACATAGTAGACAGGGAAACTCAATCAAT10.7692307692307693No Hit
GTACAGCAGTTCCTGAGCAACGATATCTCCATAATCGTGAGACAGCAGGTT10.7692307692307693No Hit
TATCAACGCAGAGTACGGGCTCTTTCCGGTGGAGGAGTCTGGAGACTGTCT10.7692307692307693No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTCTCTCGTATGCCGTC10.7692307692307693No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGATAC10.7692307692307693No Hit
GACCAGGTTGCCGTCATTGTTGCGAATCACAGCCCACATGTCCCACAGCTC10.7692307692307693No Hit
GGCATTAACAAATGTCAAGGAGTAGCAAAGTCAACAAAGATTTTAGAAGGA10.7692307692307693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.7692307692307693No Hit
GAGCAAAGGTATTGGCTGCCAAGCCTTATGATCTAAGCTGGATCCCAGAGC10.7692307692307693No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTGCAAGCGGTGGTATCAA10.7692307692307693No Hit
GCTGGTGAGATGGCTCAACAGATAAGAGCACCCGACTGTTCTTCTGAAGGT10.7692307692307693No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAATACTCTGCGTTGATACCACT10.7692307692307693No Hit
ATCATGAATCAAGCCCTGGAACAGATGAAGACAAAAGTGGATGAGTGAGTT10.7692307692307693No Hit
TCACTGATCTGTTCTGTTTTTCTTTGTTGCAAAAATGAGTACTGATATTAA10.7692307692307693No Hit
GTGTTTAGGCTCATTCTTCTTTGCATAATCTAAAGAATCATAGATCATGCC10.7692307692307693No Hit
AACGTTCACTTCTTCCACACAAATTTCCTGGTGTCCCAGCTGATCCGAGCA10.7692307692307693No Hit
GCTATATTGTTAGCACACAGAACACTTCATTACTGGGTGGGGGAAGGATCA10.7692307692307693No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAGACCTTTGGTT10.7692307692307693No Hit
GTACCACACAATTCCTGAGGGCCATCTGGAAGGCCATGGCTGCCCCCTGGC10.7692307692307693No Hit
GACCTGGACCTGCCTCTGCCTCTCAGCTGGCCTCTTAGGTCAGAGCTCTAT10.7692307692307693No Hit
ATTTGACAGTTCTAAAAACTCCTAGTCTTCCTCAATTTTAGTCTTTAATCT10.7692307692307693No Hit
GTTCACTACCATTCCACACTCAAGCACTTCTCCCATAGAGGCAGACCTAAA10.7692307692307693No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAATACTCTGCGTTTA10.7692307692307693No Hit
ATGCTTTACTGAAAGATTGATAAATCGATCAGTTACGCAAGTAGGATATAG10.7692307692307693No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
ACACAGTATCAAACTCCACTATTTGTCTGATCCGTACTTATTACAGCCGTA10.7692307692307693No Hit
CTGAATGATCGATGTCAGTCTGCCTGTGTCACCATAGCACGCTACCCACGT10.7692307692307693No Hit
GGTGAGATGATGCTGTGTGGTTAGGGAGGACAAACGCTATCTCAGCAGGAG10.7692307692307693No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTGCAAACGCGTGGTTACAACGAA10.7692307692307693No Hit
ATCTTAAATTTACCAAAGAACAAGGTCCAACTCTCTTTTGACTCCAAAATC10.7692307692307693No Hit
TAAAACAGTAGACTTAATTTTATCTAGTTTGTTGGTAAATGTGGTTCATTT10.7692307692307693No Hit
TCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCTTCCTAACATTAG10.7692307692307693No Hit
TACTTCCATATACAAAAGTCTAGCCCACTGTACCTTGAGAAGACAAAACAG10.7692307692307693No Hit
GATGAATAAAGGAGGGAATTCCCACCTCGTCACAGCTCCAGTCTCTCACAT10.7692307692307693No Hit
GCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTGCGCAAGCC10.7692307692307693No Hit
CATTTTATTCGTGTAATAGAGGGATTTCTTTATCGTATGCCGTCTTCTGCT10.7692307692307693TruSeq Adapter, Index 27 (95% over 24bp)
CAATGGATCAGAAAAGGTTCTGATAGCTCAAGAGAAAATGGCAACAAACAC10.7692307692307693No Hit
CTTATACACACTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT10.7692307692307693Illumina PCR Primer Index 11 (95% over 21bp)
CGTTAGTTAAGCTCTTGTAGAACTCTCCATATTCCTCATTAGTGATGTCAT10.7692307692307693No Hit
CCTTAGAGTACAGCACTGCAGCATCTGTGTACTCCTTTTCTTGGAATTTCC10.7692307692307693No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATATCGTAAGCCGTC10.7692307692307693No Hit
TTCTAGAACCTGCCCATCATGTCACTGGAATACCTCACCTCAAACCCCTGA10.7692307692307693No Hit
CAGGTGAAGTGCCTTGATGAAATCCTCAGGACAGACACGTCCAGGTCAGTG10.7692307692307693No Hit
GTCCCTGCTTAGCCAAAATGATGCCCCCCTTTTTATCCTTAAACATTCCAG10.7692307692307693No Hit
ATGATGGATTCTGTGTGTTTGAGAGCAATGCCATTGCCTATTATGTAAGCA10.7692307692307693No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCG10.7692307692307693No Hit
TTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTCT10.7692307692307693Illumina PCR Primer Index 11 (95% over 21bp)
CCACCACACCTCAAAGCGTGGCAATGTCAGCAACGTGGACGGGTGGGCACT10.7692307692307693No Hit
GTCTGTAAAGGATTGCTGAATCCTATTTAGTTCACAAAAATCTTCTTTGTG10.7692307692307693No Hit
TCTGACAGCTTTACTTTCCCCGTGTGATGACGGCTTCCTCTACTCCACGCT10.7692307692307693No Hit
GTAAAAGGACAGGTGAGCCGCCATGGGCTGAGCCTGCAGCTCCAACTGAAA10.7692307692307693No Hit
CCACAGAACAGCGAAAAGATCACAAGGTTAGACAACATCCACTCCAGCCTT10.7692307692307693No Hit
TTCTTATGGTTCGTCAGTGTCACTCGTGTCTTTCCAGCCACTAGATCCCAT10.7692307692307693No Hit
GACTAGAGGCGTGTGGTCCAGGTCTGCAGGTCTGGCTGGCTTTCCCTGTCT10.7692307692307693No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAAT10.7692307692307693No Hit
CCTCTGATGGCAGCTTATTTAAAACTCAGTGTGCTCCTTCACCTATACAAA10.7692307692307693No Hit
GAGCAACCGACTGCTCTTCCAAAGGTCCAGAGTTCAAATCCCAGCAACCAC10.7692307692307693No Hit
GTCTAGACTCTGTGACTAGAGTCAGCCAGAGAGCTTTGTCAAATCACAAAA10.7692307692307693No Hit
TATTCACAGTTTTGGCAGCACCGTTGCTTCATACACAGTTGGTGGCTGCCA10.7692307692307693No Hit
GGATTAATTCGATGCAACGCGAAGAACCTTACCTGGGTTTGACATACACCG10.7692307692307693No Hit
CTCTTACCACTGAGCCATCTCTCCAGCCCCAAAACCTTACTTTTCTTATAG10.7692307692307693No Hit
TGTCAGACATCTACAACATTTTGTTGTAAAAAAGTCACACGATCATGTCAT10.7692307692307693No Hit
GTGCGTGTGTGTGTGTCGTATCAGTATGGGAGGTGCCTCTTTGAGTTCTCT10.7692307692307693No Hit
CTTGTTCACTGTATCAAAGTCAATAGTGATGCCGAAAGCCACGCCCATCTC10.7692307692307693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTAAGGTGGTT10.7692307692307693No Hit
GGATGAGTAAAAGTGACAGAAAAGTTAGGTGAGTGACCAGAGAGTAAAGCC10.7692307692307693No Hit
CCCTCACAGGGGCAGAAGGCTGAAGAACAGGAGCCAGAACTGACATCAACT10.7692307692307693No Hit
GAAGGGAGCTGTGGATGGAGGCTTGTCTATCCCTCATAGTACCAAACGATT10.7692307692307693No Hit
AACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT10.7692307692307693No Hit
TGCTTCAACAGTGTTTGAACGGAACAGACCCGGGGATTCCCACTGTACTCG10.7692307692307693No Hit
ACCTATAGTAAAGACACTGCCCATCCTGCTGAGTATTTTTCATCTGTAGTT10.7692307692307693No Hit
CTTTCACCCTGATCTTTTCCTTCTGTTCCCCTGTAACTTTTTAATCACCTT10.7692307692307693No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers