FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_78.34100000007371.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_78.34100000007371.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257521
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC31281.2146582220479107No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT3060.11882526085251299Illumina PCR Primer Index 8 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4150.036.8639745
TATGCCG4250.035.99658243
ATGCCGT4350.035.16907544
ATCTCGT4300.035.0548137
CTCGTAT4250.034.9378639
CGAGACT4250.034.9378623
AATCTCG4200.034.81812336
CGTATGC4450.034.3787641
TCGTATG4450.034.3787640
ACGAGAC4400.034.25811422
TCTCGTA4350.034.1346938
CACGAGA4450.033.87318821
CCCACGA4550.033.6231819
CCACGAG4550.033.6231820
GACTTGC4500.032.99686426
GCCCACG4650.032.90010518
CTCCGAG4550.032.64694212
TCCGAGC4550.032.64694213
TCTCCGA4600.032.2983611
CGAGCCC4600.032.29208415
Produced by FastQC (version 0.11.2)