FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_76.34100000007358.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_76.34100000007358.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328699
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC35581.0824492925138196No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT3920.11925804459399025Illumina PCR Primer Index 8 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4300.040.289239
TCTCGTA4400.039.37353538
CGTATGC4450.038.93113741
TGCCGTC4450.038.93113745
AATCTCG4350.038.7916636
TCGTATG4500.038.4985740
ATCTCGT4550.038.0755137
CCCACGA4500.037.9985919
CACGAGA4450.037.91993721
ACGAGAC4450.037.91993722
GCCCACG4600.037.66164418
CTCCGAG4600.037.66164412
CGAGACT4500.037.49860423
TATGCCG4650.037.25667643
TCCGAGC4700.036.86033213
CGAGCCC4700.036.86033215
TCTCCGA4700.036.86033211
CCACGAG4600.036.6834220
ATCTCCG4800.036.56114210
ATGCCGT4850.035.72031844
Produced by FastQC (version 0.11.2)