FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_75.3410000000734b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_75.3410000000734b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124867
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC42523.405223157439516No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT5980.478909559771597Illumina PCR Primer Index 8 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4760.3812056027613381No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.14014911866225666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT800.039.37219245
TGCCGTC5550.038.10539645
ATCTCGT5600.036.96165537
CGAGCCC5850.036.9352415
CGAGACT5850.036.9204523
CCACGAG5850.036.9204520
TATGCCG5800.036.85082243
TCGTATG5800.036.85082240
GACTTGC5750.036.77998726
ATGCCGT5750.036.77998744
TCTCGTA5700.036.70791638
ACGAGAC5950.036.67806222
GAGCCCA5900.036.6222316
CACGAGA5900.036.60756321
CCCACGA5900.036.60756319
AATCTCG5550.036.4838936
CTCGTAT5750.036.3887139
GCCCACG5950.036.3144818
TCCGAGC5950.036.3144813
CCGAGCC5950.036.3144814
Produced by FastQC (version 0.11.2)