FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_74.3410000000733e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_74.3410000000733e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575264
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC12800.22250653612949878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC1700.031.7634822
CTCGTAT1650.031.36242539
AATCTCG1700.030.44000236
CGAGCCC1800.029.9988415
CGTATGC1750.029.57028841
TATGCCG1750.029.57028843
ATCTCCG1850.029.19059810
CACGAGA1850.029.1880621
TCGTATG1950.028.8450440
GCCCACG1900.028.41995418
GTATGCC2000.026.99895742
CCACGAG2000.026.99895720
CGAGACT1950.026.53743623
CCCACGA2100.025.71329319
ATGCCGT2050.025.24292644
TCTCGTA2200.023.52181838
TCTCCGA2300.023.47939511
ATCTCGT2300.023.47735437
TCCGAGC2350.022.97783513
CCGAGCC2400.022.49913214
Produced by FastQC (version 0.11.2)