FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_72.34100000007314.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_72.34100000007314.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124521
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC3120.2505601464813164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG253.8707287E-545.043
CGCTATA253.8707287E-545.031
CGAGCCC356.203525E-638.57142615
GCCCACG301.1340852E-437.50000418
CTCGTAT301.1340852E-437.50000439
CACGAGA301.1340852E-437.50000421
GAGACGA301.1340852E-437.50000424
ACGCTAT301.1340852E-437.50000430
CGAGACG301.1340852E-437.50000423
ATGCCGT301.1340852E-437.50000444
CGTAAAC250.00209991536.027
CCTATAC250.00209991536.03
GCTATAT401.5494594E-533.75000432
CCCACGA401.5494594E-533.75000419
TCGTATG401.5494594E-533.75000440
ATCTCCG352.8060493E-432.14285710
CGACGCT352.8060493E-432.14285728
CTATATC352.8060493E-432.14285733
AGACGAC352.8060493E-432.14285725
GACGCTA352.8060493E-432.14285729