FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_71.34100000007307.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_71.34100000007307.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241296
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC13120.5437305218486838No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2990.12391419667130826No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCC352.814047E-432.1424144
AGACGAC1550.030.4834525
TGCCGTC1600.029.53696345
TATCTCG1600.029.5308436
CCGAGCC1600.029.5308414
ACGAGAC1600.029.5308422
ACGACGC1600.029.5308427
GCCGTCT554.1407893E-628.64190345
CGACGCT1650.028.6359728
ACGCTAT1650.028.6359730
CGAGCCC1650.028.6359715
CGAGACG1650.028.6359723
CTCGTAT1600.028.1246139
ATAGACC406.1524153E-428.124613
TATGCCG1600.028.1246143
GTATGCC1600.028.1246142
ATGCCGT1600.028.1246144
CACGAGA1700.027.79373421
GAGACGA1700.027.79373424
CGCTATA1700.027.79373431
Produced by FastQC (version 0.11.2)