FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_70.341000000072f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_70.341000000072f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC77.142857142857142No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC66.122448979591836No Hit
AAAGAGGTACTGTCAAAGCGATAAAAATTCATTAGCATGGTGATTATATTT11.0204081632653061No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGACAGAGAGAGACAGA11.0204081632653061No Hit
ACACTGCCCTCCTACATCCTGAAAAGAAGGGATATGGCCCAAGACAGAGGA11.0204081632653061No Hit
CAGTAAGTGAATGGTCCCTTCTGCAACTACTGAAACAAATTAGCTACATTG11.0204081632653061No Hit
ACTCAGGGCATGGACGCGACCATCCTCCTCTTAGGAGTGGGGGTGGCTTTT11.0204081632653061No Hit
AAATTACACACTATCCATATAGACAGATTTTGGTTACACTTTGCCTATTGA11.0204081632653061No Hit
CCTTGAAGCTACCAAAAGAAGATGATAGTGGCAACCTAAAGCCTTTGGAGT11.0204081632653061No Hit
GTACAAACAAGCCAGGAATCTGCAGGGTGCCCACCTAACAGGCCGAGGAGA11.0204081632653061No Hit
TTCCAGTTCCAGTCCAGAAGAACATTGATCAGCAGATTAAAACCTGTCTTT11.0204081632653061No Hit
GAAGGAGGCTGCTGAGATGGGAAAGGGCTCCTTCAAGTACGCCTGGGTCTT11.0204081632653061No Hit
CCTTTGTGTTGGATGAGTTTAAGCGTAAGTACTCCAATGAGGACACCCTCT11.0204081632653061No Hit
ACTTTCATCTGAGTCTAGAGATTTCTTTTCCTTTTTTGGGTCACCTGAAGC11.0204081632653061No Hit
GAAACATGGTGGCCCGGCGGATGAAGAGAGGCATGTTGGAGACCTGGGCAA11.0204081632653061No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTG11.0204081632653061No Hit
GTATCTCTGTTCTCTGGGTGAACTTTTGCCTCTGTAGTTCCTTTTCTGTCT11.0204081632653061No Hit
CAAGTACACCAAGGGAATGAGCCAACTTGAAAAATTAGTAATGCTGTGTCT11.0204081632653061No Hit
CTTATACACATCTCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT11.0204081632653061No Hit
TCCCAAGTCCATGGGATCTTTTGCTGACCTGCTTTTCAGATCCGTATCTCC11.0204081632653061No Hit
CTTCCTGGATAAGGAGGTGAAACTCATCAAGAAGATGGGCAACCATCTGAC11.0204081632653061No Hit
GCAGTTATCTGGTCCTTACTCAACAGTTGTTTAAAACTCAAAAACTGTTAG11.0204081632653061No Hit
TACCAATATGATGAGACATCTGGCTACTACTATGACCCCCAGACCTGTCTC11.0204081632653061No Hit
TTCACTTCCCCAAGACTGACATTGACAACAAATTTCCTTTGGCCTGTCTCT11.0204081632653061No Hit
GGTTGAAACAGCTCAGGAAAGAAATGAGACTCTCTTTCCAGCTCTTATCTA11.0204081632653061No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT11.0204081632653061RNA PCR Primer, Index 44 (95% over 22bp)
AGTCACGAGGACTCAAGGAACCAAAATTGCCTCTGATGGCCTCAAGGGTCG11.0204081632653061No Hit
CCCTCTTCTCACTCTGCGTCGCTTTCCTCCTTGTGCAGGTAGGAGTGTTGC11.0204081632653061No Hit
GCCTTTAAGAAAACCTCTACAAACTCTTTCAAAAACCCATAGAAATCTAAA11.0204081632653061No Hit
TCTTTGGAGTTGGCCCCCGTCTATGTTCCTTGGAACAGGACGTCACAGAGG11.0204081632653061No Hit
GGTCATGTGTGTGGTGCAGGACAGCTGCTCTGGGTCAGCAGAGTAAACATT11.0204081632653061No Hit
GCACAGATGCGGGAGCCAAAAGCTGCAATGCAAAGCGCAGCGAAGTTCGCT11.0204081632653061No Hit
GTAGTAGCTGCACACCTTTAATCCCAGGCAGAGGCAGCTGAATCTCTGTGA11.0204081632653061No Hit
CCGCTGCGTGGTCCTGACCGGCGCCGGGCGGGCATTCTCGGCCGGCGGCGA11.0204081632653061No Hit
CTTTTAGGCAGAATTATCAATCTGAAGATCCACAATCTTTTATAAAAGGAA11.0204081632653061No Hit
GTGTGTAGTTCCTCAAGACAGCCAGGGCTATACAGAGAAACCCTGTCTCAA11.0204081632653061No Hit
TCTTAGCATGGAGGGACAAAGAAAGCTGTACATTGTTGCTTGAGAGTATGT11.0204081632653061No Hit
AAACCTGGTCTAGTATTTAAGACCACAGCTTTTGAAGTTCTCACTTTTCTT11.0204081632653061No Hit
GTACATGGACATACTATGCACACGAGCAAAGTCCATTCATGGGTGAAAGTC11.0204081632653061No Hit
AGTGTGGTCTGTTGCTTTGTGTTTCCTTGTAAGCTGAGACTGAGCGAATGC11.0204081632653061No Hit
CTCCAGTCACCGGTAGCCTTGGCAATGTCATCGCCAACTTTCTTCGGAGAC11.0204081632653061No Hit
CCTCTAGCCAGGCCACACCTCCTAATAATGCCAGTCTGTATGGGCCAAGCA11.0204081632653061No Hit
CTATGTTTCAATTAGCAATTTTCTGAATCATTACTTGCCTATAGTCCAGCA11.0204081632653061No Hit
GCCCTGATGCACACGAACATGCGGCTGAGGCCCGCGTGGGAAAAGCGGATG11.0204081632653061No Hit
CAATATTAGGACTGCTGTTTAAGATTTGTCTCTGGTCAAATCAAGCCCTGT11.0204081632653061No Hit
CATGAGGACATAGAGACAGGAGGATGGCATCATGAAGCCAGTATGGGTTAC11.0204081632653061No Hit
ATCTAGAGAGAGGTTCTTGATTTGGTTTTGGTGCCATATTTGGGTTTTAAT11.0204081632653061No Hit
TGCTAATAATTGGCTGTGGACGCTGAATCCGTCTCCGGCATATGTTGGACA11.0204081632653061No Hit
CTCCTTTATAGCCAAACTCGGTGAGCAGCTCCCGGATCTCCAGCTCGACTA11.0204081632653061No Hit
CCACAAACAAAAAAAAAAAAGGGAAAAAAGACAAAGACCAAAAAAAACCAA11.0204081632653061No Hit
GGGTAACTTCCTGAAGGCTACGTTCGAGGCTGTGTCGAAGACGTACGCTGT11.0204081632653061No Hit
GCCATAGACAAGATGCAGATGAATACCAACCAACTGACCTCAGTACATGCC11.0204081632653061No Hit
ATGACGAAATCACTGAAAAACGTGAAAAATGAGAAATGCACACTGTAGCTG11.0204081632653061No Hit
CCTATAAACTCAACTGCTTAGGAGCCTGAGCTGGGACTGTCTCTTATACAC11.0204081632653061No Hit
CCTCTTTGATTGTCTCATAGCTTGGAGGAAGCCCGGCATTGGTGCTGTCTC11.0204081632653061No Hit
ATGCTGAACCCTGGCCTACCCAGCACCACATAGAAGTCCAGGCCGTAGATC11.0204081632653061No Hit
GAACAAAATTTGCTAATATCAATTAATGACCTGCATGCTTTTTCTTTACCC11.0204081632653061No Hit
CCCCCGACCTCTGGAAAGAGACTGTCTTCACCAAGTCTCCTTATCAGGAAT11.0204081632653061No Hit
GTATAAAGAGGAAGGCTTAAATGCGTTCTACAAGGGCGTTGCTCCTCTGTG11.0204081632653061No Hit
CTTATACACATTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT11.0204081632653061Illumina PCR Primer Index 8 (95% over 21bp)
GGCTGAGAGAGACCCTTCTGGATCGATTCATACAGAGCCCAAGCTGAGAAA11.0204081632653061No Hit
GATGGAAAGGAAGGTCAGCCTGAGGAAGATGGGAAGGAAATTCATCATGAG11.0204081632653061No Hit
GAACTCAATGACTAGAAATATTGGTGAAGCTTTTAATGTTTTCTTCGTCTA11.0204081632653061No Hit
CTATGAGGCATACAGTAAGTTTCTTGCTTTTCTTTTTGAGGTATGTACTGT11.0204081632653061No Hit
AGTTCAGGCTGCCCTCAAATTCCAGATGGCAGTGCTGGCCGGGAACATTAG11.0204081632653061No Hit
CAGTAGACCTGGAACGTCACCTCGCCGCGATCGCCTGGAGAAATGACCGCC11.0204081632653061No Hit
GTATTTCTCATTATCCGTGATTTTCAGTTTTCTCGCCATATTAAGGTTCCT11.0204081632653061No Hit
CTCCAAGGCCTACCACCTTCAGAAGTCGACTTGTGGCAAGTGTGGCTACCC11.0204081632653061No Hit
AAATTTACCACCTTTTTCAGTTTTCTTGCCATATTCCACATCCTACAGTGG11.0204081632653061No Hit
GGATGCTAGCTGAAAGACTGTGATCCCGCTGACTGTTCCCTCGCCCACCTG11.0204081632653061No Hit
TACCACAAGAGGTTGATATTTTCATAGTGGCGTGTAATCTCCTTTTCAGGA11.0204081632653061No Hit
TGCTGAAATAATAGAAAGTGACTGCTTGTCAGGTGTAAGTGATCAACAATT11.0204081632653061No Hit
TTGCAGCGGCGCGGGCGAGCGGAAAGGCCGCGGGAGGCGGCGAGCCAGCGG11.0204081632653061No Hit
ACAGAGCTCCTTTTCCTTTTCATTGAGTTTCTCTGTACCAGGGAGGCCCGT11.0204081632653061No Hit
CTGAAAGATAAAGGAAACTGGAAGTCACATAAAAACAAAACCCACAATGTC11.0204081632653061No Hit
GGCTCAGATTGAACGCTGGCGGCATGCTTTACACATGCAAGTCGGACGGCA11.0204081632653061No Hit
GGGGTAGACAGGCTGGAGCTTCCCACAGCATCAGACTTCAGAGACCACATT11.0204081632653061No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTCTTTTTCTTTTACA11.0204081632653061No Hit
GGTCAGTGAACACCTTACCAGACCAGCCAGTCACCAAGTGACCTGCTCTTC11.0204081632653061No Hit
GCACAAAGCAACCCCTCTGCAAATTACTCCTGATGAAGATGAAAAGCCAGC11.0204081632653061No Hit
GTGGAAAAACAGTAAAGTTGCCCTGGACTTTAACTCAAATGAATTGCTCTA11.0204081632653061No Hit
GTATATATCTAGGAGGGAAGTTGAAGAAGTTTGTAATGGTGGTAATGCTGT11.0204081632653061No Hit
GCACCCGACTGCTCTTCCGAAGGTCCAGAGTTCAAATCCCAGCAACCACAT11.0204081632653061No Hit
TAGAAGATGTATAAGTTGATCGTAACGGAAGCGTGGATAAGATGCTCGGAT11.0204081632653061No Hit
GACCCTCTCCCCATCCCGTCAGTCACCTGACCCTGTGGAAGGAGCAACCGA11.0204081632653061No Hit
GTCTTAAGCTTTGTCCTGTGTTCCTTTCTTTGTTACCGATTTAATTCTATC11.0204081632653061No Hit
CATATAGTGTGTGCTTTGATCCGTGCCATGGGAGCTCAGACACTGCCTGTG11.0204081632653061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGC50.045.018
CTTTTCA50.045.032
TTTTGCT50.045.019
ATGGGAT50.045.011
ATCTTTT50.045.016
TCCCAAG50.045.01
TGACCTG50.045.025
CCTGCTT50.045.028
GTATCTC50.045.044
GGATCTT50.045.014
TTTTCAG50.045.033
TGCTGAC50.045.022
CCATGGG50.045.09
TTTCAGA50.045.034
CTGACCT50.045.024
ACCTGCT50.045.027
CCGTATC50.045.042
CCCAAGT50.045.02
TGCTTTT50.045.030
TATCTCC50.045.045
Produced by FastQC (version 0.11.2)