FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 12h_7.34100000006ee3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 12h_7.34100000006ee3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631353
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGAAT250.002106481535.99968726
TATCTCG300.005145743529.99973736
CGAATTG300.005145743529.99973728
GTATCAA3150.027.8679331
TAACGTC500.002263603322.49980444
TCAACGC4050.018.8887214
GTTCTAG1555.293259E-1018.8782771
AGTCGTC600.00650765318.7498368
CAACGCA4250.017.9998425
ATCAACG4400.017.386213
TATGGCG2251.8189894E-1216.9998516
GTATAGG1205.146654E-616.8815361
TATCAAC5000.016.6498532
AACGCAG4600.016.630296
CTAATAC952.8170194E-416.5788023
GTACTAA1004.1510805E-415.7561021
GGTATCA2202.1464075E-1015.346851
TTAGACA1209.741811E-514.9998684
CTAGTAC900.003751644914.9998673
ATATACT900.003751644914.9998674
Produced by FastQC (version 0.11.2)